Abstract
Circular RNA (circRNA) has been confirmed to regulate breast cancer (BC) progression. However, the role of circ_0059457 in BC progression is still unclear.The expression of circ_0059457, taspase 1 (TASP1), microRNA (miR)-140-3p and ubiquitin-binding enzyme E2C (UBE2C) was detected by quantitative real-time PCR. Cell proliferation, migration, invasion and sphere formation ability were assessed by cell counting kit-8 assay, EdU assay, wound healing assay, transwell assay and sphere formation assay. Cell glycolysis was assessed by detecting glucose uptake, lactate levels and ATP/ADP ratio. Dual-luciferase reporter assay, RIP assay, RNA pull-down assay were used to validate RNA interaction. Xenograft tumor model to assess the effect of circ_0059457 on BC tumor growth in vivo. Circ_0059457 had elevated expression in BC tissues and cells. Circ_0059457 knockdown inhibited BC cell proliferation, metastasis, sphere formation ability, and glycolysis. In terms of mechanism, circ_0059457 sponged miR-140-3p, and miR-140-3p targeted UBE2C. MiR-140-3p inhibition reversed the effect of circ_0059457 knockdown on BC cell malignant behaviors. Besides, miR-140-3p overexpression inhibited BC cell proliferation, metastasis, sphere formation ability and glycolysis, and these effects were abrogated by UBE2C enhancement. Furthermore, circ_0059457 regulated UBE2C expression through sponging miR-140-3p. Additionally, circ_0059457 knockdown obviously inhibited BC tumor growth in vivo. Circ_0059457 promoted BC progression via miR-140-3p/UBE2C axis, which provided potential target for the treatment of BC.
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LH: conceived and designed the study, and drafted the first draft of the manuscript. All experiments were completed by all authors. GZ, LH, XB, ZX, FW, GH: analyzed and collated the results. All authors reviewed and critiqued the manuscript, and agreed to the final submission of the manuscript. All authors read and approved the final manuscript.
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Written informed consents were obtained from all participants and this study was permitted by the Ethics Committee of Shanxi Province Cancer Hospital, Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Cancer Hospital Affiliated to Shanxi Medical Univsersity.
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10528_2023_10407_MOESM1_ESM.tif
Supplementary material 1 (TIF 408.8 kb) Circ_0059457 knockdown inhibited ECAR level in BC cells. MB231 and BT549 cells were transfected with sh-circ_0059457 or sh-NC. The ECAR level was detected by ECAR Kit in MB231 cells (A) and BT549 cells (B).
10528_2023_10407_MOESM2_ESM.tif
Supplementary material 2 (TIF 255.5 kb) Circ_0059457 knockdown reduced EMT in BC cells. MB231 and BT549 cells were transfected with sh-circ_0059457 or sh-NC. The levels of E-cadherin and N-cadherin were detected by WB analysis in MB231 cells (A) and BT549 cells (B). *P < 0.05.
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Huang, L., Zhang, G., Han, L. et al. Circ_0059457 Promotes Proliferation, Metastasis, Sphere Formation and Glycolysis in Breast Cancer Cells by Sponging miR-140-3p to Regulate UBE2C. Biochem Genet 62, 125–143 (2024). https://doi.org/10.1007/s10528-023-10407-8
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DOI: https://doi.org/10.1007/s10528-023-10407-8