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Assessment of ACC and P450 Genes Expression in Wild Oat (Avena ludoviciana) in Different Tissues Under Herbicide Application

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Abstract

Target-site resistance (TSR) and non-target-site resistance (NTSR) to herbicides in arable weeds are increasing rapidly all over the world and threatening universal food safety. Resistance to herbicides that inhibit ACCase activity has been identified in wild oat. In this study, expression of ACC1, ACC2, CYP71R4 and CYP81B1 genes under herbicide stress conditions were studied in two TSR (resistant in the residue Ile1781-Leu and Ile2041-Asn of ACCase) biotypes, two NTSR biotypes and one susceptible biotype of A. ludoviciana for the first time. Treated and untreated biotypes with ACCase-inhibitor clodinafop propargyl herbicide were sampled from the stem and leaf tissues at 24 h after treatment. Our results showed an increase in gene expression levels in different tissues of both types of resistance biotypes that occurred under herbicide treatment compared with non-herbicide treatment. In all samples, the expression levels of leaf tissue in all studied genes were higher than in stem tissue. The results of ACC gene expression showed that the expression level of ACC1 was significantly higher than that of ACC2. Also, expression levels of TSR biotypes were higher than NTSR biotypes for the ACC1 gene. For both CYP71R4 and CYP81B1 genes, the expression ratio increased significantly in TSR and NTSR biotypes in different tissues after herbicide treatment. In contrast, the expression levels of CYP genes in NTSR biotypes were higher than in TSR biotypes. Our results support the hypothesis that the reaction of plants to herbicide is carried out through a different regulation of genes, which can be the result of the interaction of resistance type in the target or non-target-site.

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Abbreviations

ACCase:

Acetyl-CoA carboxylase

GTs:

Glucosyltransferases

GSTs:

Glutathione S-transferases

TSR:

Target-site resistance

NTSR:

Non-target-site resistance

CYP:

Cytochrome P450 monooxygenase

qPCR:

Quantitative polymerase chain reaction

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Acknowledgements

Lorestan University, Khorramabad, Iran, supports this project.

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Lorestan University, Khorramabad, Iran.

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Authors and Affiliations

  1. Department of Plant Production and Genetic Engineering, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

    Ali Akbarabadi, Ahmad Ismaili & Farhad Nazarian Firouzabadi

  2. Department of Horticulture, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey

    Sezai Ercisli

  3. Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Danial Kahrizi

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  1. Ali Akbarabadi
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Software, AA; Supervision, AI and DK; Writing, review and editing, FNF and SE; Research, AA; All authors have read and agreed to the published version of the manuscript.

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Correspondence to Danial Kahrizi.

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Akbarabadi, A., Ismaili, A., Nazarian Firouzabadi, F. et al. Assessment of ACC and P450 Genes Expression in Wild Oat (Avena ludoviciana) in Different Tissues Under Herbicide Application. Biochem Genet 61, 1867–1879 (2023). https://doi.org/10.1007/s10528-023-10357-1

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