Abstract
Terpene synthases (TPSs) catalyze terpenoid synthesis and affect the intracellular isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) concentration. In this study, we mined the in silico genome-wide TPS genes of Hevea brasiliensis and identified 47 full-length TPS genes. They had DDXXD, DXDD, NSE/DTE, RR(X)8 W, EA(X)W, and other conserved motifs. The phylogenetic tree analysis revealed that the TPSs of H.brasiliensis (HbTPSs) were divided into five subfamilies, TPS-a, TPS-b, TPS-c, TPS-e/f, and TPS-g. HbTPSs were predicted to have functions in the cellular components, molecular functions, and biological processes. HbTPSs were involved in seven pathways, which were K14173, K14175, K15803, K04120, K04121, K17982, and K12742 in the secondary metabolite pathway prediction. Three-dimensional structures of HbTPSs of 7 pathways were predicted, and DDXXD, NSE/DTE, and EA(X)W conserved motifs near the binding sites were found. Cis-acting elements analysis showed that they had more cis-acting elements related to phytohormone responsiveness, which indicated that terpenoid biosynthesis might be related to phytohormone regulation. RNA-Seq analysis showed that different HbTPSs were expressed differentially in different tissues. This study’s results help reveal the role of HbTPSs and their molecular mechanism and help resolve the regulatory mechanism of terpenoid biosynthesis in H.brasiliensis.
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Data Availability
The RNA-seq can download from NCBI SRA database (http://www.ncbi.nlm.nih.gov/sra/) under the accession number PRJNA742874. The genome sequence and annotation file can download from NCBI Genome database (http://www.ncbi.nlm.nih.gov/genome/) under the accession number ASM165405v1. Other datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
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This work was funded by Guangzhou Municipal Science and Technology Project (Grant No. 201903010025) and Science and Technology Planning Project of Guangdong Province (Grant No. 2016A010105020).
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JHL and MHF contributed to the study’s conception and design. JHL, DW, XL, WWH, and CX contributed to the analysis of the data. JHL and MHF wrote the paper. MHF, HMZ, and QM revised the paper. All authors read and approved the final version of the manuscript.
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Liang, J., Wang, D., Li, X. et al. In Silico Genome-Wide Mining and Analysis of Terpene Synthase Gene Family in Hevea Brasiliensis. Biochem Genet 61, 1185–1209 (2023). https://doi.org/10.1007/s10528-022-10311-7
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DOI: https://doi.org/10.1007/s10528-022-10311-7