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A Study of Combined Genotype Effects of SHCBP1 on Wool Quality Traits in Chinese Merino

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Abstract

SHCBP1 (Shc SH2-domain binding protein 1) is a member of the Src and collagen homolog (Shc) protein family and is closely associated with multiple signaling pathways that play important roles during hair follicle induction, morphogenesis, and cycling. The purpose of this study was to investigate SHCBP1 gene expression, polymorphisms, and the association between SHCBP1 and wool quality traits in Chinese Merino sheep. The SHCBP1 gene was shown, by qPCR, to be ubiquitously expressed in sheep tissues and differentially expressed in the adult skin of Chinese Merino and Suffolk sheep. Four SNPs (termed SHCBP1SNPs 1–4) were identified by Sanger sequencing and were located in exon 2, intron 9, intron 12, and exon 13 of the sheep SHCBP1 gene, respectively. SHCBP1SNPs 3 and 4 (rs411176240 and rs160910635) were significantly associated with wool crimp (P < 0.05). The combined polymorphism (SHCBP1SNP3-SHCBP1SNP4) was significantly associated with wool crimp (P < 0.05). Bioinformatics analysis showed that the SNPs associated with wool crimp (SHCBP1SNPs 3 and 4) might affect the pre-mRNA splicing by creating binding sites for serine–arginine-rich proteins and that SHCBP1SNP4 might alter the SHCBP1 mRNA and protein secondary structure. Our results suggest that SHCBP1 influences wool crimp and SHCBP1SNPs 3 and 4 might be useful markers for marker-assisted selection and sheep breeding.

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Funding

This work was supported by Young and Middle-aged Scientific and Technological Innovation Leading Talent Plan of the Xinjiang Production and Construction Corps (Grant No. 2019CB019), Young and Middle-aged Talents Training and Guidance Plan of State Key Laboratory of Sheep Genetic Improvement and Healthy Production (No. SKLSGIHP2016A01), and Major Scientific and Technological Project of the Xinjiang Production and Construction Corps (No. 2017AA006), China.

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Authors and Affiliations

  1. Ministry of Education, Key Laboratory for Ecology of Tropical Islands; Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, 571158, People’s Republic of China

    Guang-Wei Ma

  2. State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, 832000, People’s Republic of China

    Hua Yang

  3. Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Guang-Wei Ma, Shou-Zhi Wang, Ning Wang & Hui Li

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  1. Guang-Wei Ma
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  2. Shou-Zhi Wang
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  3. Ning Wang
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  4. Hui Li
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  5. Hua Yang
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Contributions

GWM, HY, NW, and HL designed and conceived the experiment. GWM, HY, and SZW performed the data analysis and draft the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hua Yang.

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Ethical Approval

This study was carried out in accordance with the Guidelines for the Care and Use of Laboratory Animals and approved by the Experimental Animal Care and Use Committee of Xinjiang Academy of Agricultural and Reclamation Sciences (Shihezi, China, ethic committee approval number: XJNKKXY-AEP-039, 22 January 2012).

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10528_2022_10268_MOESM1_ESM.docx

Supplementary Table S1 Allele and genotype frequencies of the identified SNPs of SHCBP1 in Chinese Merino sheep (Junken Type) (DOCX 19 kb)

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Ma, GW., Wang, SZ., Wang, N. et al. A Study of Combined Genotype Effects of SHCBP1 on Wool Quality Traits in Chinese Merino. Biochem Genet 61, 551–564 (2023). https://doi.org/10.1007/s10528-022-10268-7

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  • DOI: https://doi.org/10.1007/s10528-022-10268-7

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