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Circ_HIPK3 Inhibits H2O2-Induced Lens Epithelial Cell Injury in Age-Related Cataract Depending on the Regulation of miR-495-3p/HDAC4 Pathway

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Abstract

Age-related cataract (ARC) is one of the most common chronic diseases. Circular RNA (circ)_HIPK3 is reported to be involved in the advancement of ARC, but its molecular mechanism has not been clarified. Our study provides a new perspective on the clinical treatment of ARC. Our data showed that the expression levels of circ_HIPK3 and histone deacetylase 4 (HDAC4) were downregulated, while microRNA (miR)-495-3p level was increased in ARC tissues and H2O2-induced SRA01/04 cells. Functional experiments showed that circ_HIPK3 and HDAC4 overexpression could inhibit H2O2-induced lens epithelial cell apoptosis and fibrosis. In terms of mechanism, we found that circ_HIPK3 could sponge miR-495-3p, miR-495-3p could target HDAC4. Besides, we confirmed that circ_HIPK3 sponged miR-495-3p to positively regulate HDAC4. Additionally, miR-495-3p overexpression or HDAC4 knockdown reversed the inhibition effect of circ_HIPK3 on H2O2-induced lens epithelial cell injury. In conclusion, our data showed that circ_HIPK3 suppressed H2O2-induced lens epithelial cell injury by regulating miR-495-3p/HDAC4 axis.

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Data Availability

The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.

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Author notes

  1. Sihui Chen and Minghong Wang are Co-first authors.

Authors and Affiliations

  1. Department of Ophthalmology, Jingmen No.1 People’s Hospital, No. 168, Xiangshan Avenue, Duodao District, Jingmen, 448000, China

    Sihui Chen, Minghong Wang, Rui Jian, Hui Li, Guoli Liu, Cuiyun Zhou, Yan Xiong & Wenqian Wang

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  1. Sihui Chen
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  2. Minghong Wang
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  3. Rui Jian
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Correspondence to Wenqian Wang.

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The design of this protocol follows the tenets of the Declaration of Helsinki, approved by the Ethics Committee of Jingmen No.1 people’s Hospital.

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Chen, S., Wang, M., Jian, R. et al. Circ_HIPK3 Inhibits H2O2-Induced Lens Epithelial Cell Injury in Age-Related Cataract Depending on the Regulation of miR-495-3p/HDAC4 Pathway. Biochem Genet 61, 565–577 (2023). https://doi.org/10.1007/s10528-022-10266-9

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  • DOI: https://doi.org/10.1007/s10528-022-10266-9

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