Abstract
Circular RNA (circRNA) 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (circATIC; hsa_circ_0058058) was observed to be upregulated in multiple myeloma (MM) by former article. However, the function and exact mechanism of circATIC in MM development remain barely known. CircRNA-microRNA (miRNA)-messenger RNA (mRNA) axis was established through using bioinformatic databases (starbase, Circinteractome, and microT-CDS). Dual-luciferase reporter assay, RNA immunoprecipitation assay, and RNA-pull down assay were utilized to verify the target relationship between microRNA-324-5p (miR-324-5p) and circATIC or hepatocyte growth factor (HGF). CircATIC expression was upregulated in MM patients and cell lines. CircATIC interference notably hampered cell proliferation, migration, invasion, and glycolysis and induced cell apoptosis of MM cells. MiR-324-5p was a target of circATIC. CircATIC silencing-mediated effects in MM cells were largely overturned by the knockdown of miR-324-5p. HGF was a target of miR-324-5p, and circATIC upregulated the expression of HGF partly through sponging miR-324-5p in MM cells. MiR-324-5p suppressed the malignant behaviors of MM cells, which were largely counteracted by the overexpression of HGF in MM cells. CircATIC accelerated the proliferation, migration, invasion, and glycolysis and suppressed the apoptosis of MM cells through mediating miR-324-5p/HGF signaling.
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The analyzed datasets generated during the present study are available from the corresponding author on reasonable request.
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Conceptualization and methodology were performed by FW and YW, formal analysis and data curation were performed by YW, XD, and WW, validation and investigation were performed by BW and FW, writing—original draft preparation and writing—review and editing were performed by BW, FW, and YW, and approval of final manuscript was performed by all authors.
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Wu, B., Wang, F., Wang, Y. et al. CircATIC Contributes to Multiple Myeloma Progression via miR-324-5p-Dependent Regulation of HGF. Biochem Genet 60, 2515–2532 (2022). https://doi.org/10.1007/s10528-022-10228-1
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DOI: https://doi.org/10.1007/s10528-022-10228-1