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circ3323 Motivates Host Gene to Promote the Aggressiveness of Bladder Cancer

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Abstract

Bladder cancer (BCa) is the most common cancer in the urinary system with high recurrence rate and poor prognosis. Circular RNA (circRNA) is a novel subclass of noncoding-RNA which participate in progression of BCa. Here, we identified a novel circRNA–circ3323 and aimed to investigate the role of circ3323 in progression of BCa. Public data of RNA sequencing was used to identify significant circRNA related to BCa. The role of circRNAs in progression of BCa was assessed in cytotoxicity assay, transwell assay and flow cytometry. Biotin-coupled RNA pull-down and fluorescence in situ hybridization were performed to evaluate the interaction between circRNAs and miRNAs. The expression of circ3323 was higher in BCa tissues and cells than in normal samples. Experiments in vitro showed that the knockdown of circ3323 inhibited cell proliferation and impeded the metastasis of BCa cells. Mechanistically, we demonstrated that circ3323 acts as a sponge for miR-186-5p and promotes host gene APP’s expression. Clinically, circ3323 predicts worse overall survival of BCa patients, indicating its prognostic value. Our study identified that circ3323 modulates metastasis of BCa through miR-186-5p/APP axis and may serve as a promising prognostic biomarker for BCa, which provides novel insights into treatment of BCa.

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Funding

Funding was provided by Changzhou Science and Technology Bureau (WZ201931).

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Authors and Affiliations

  1. Department of Urology, The Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Xinglong Road 29, Tianning, Changzhou, 213000, China

    Xingyu Wu, Wei Cao, Chao Lu, Li Zuo & Minjun Qi

  2. Department of Urology Surgery, Changzhou Wujin People’s Hospital, Wujin Hospital Affiliated Jiangsu University, The Wujin Clinical College of Xuzhou Medical University, Changzhou, China

    Xiaowu Liu

Authors
  1. Xingyu Wu
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  2. Wei Cao
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  3. Chao Lu
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  4. Li Zuo
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  5. Xiaowu Liu
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  6. Minjun Qi
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Contributions

WXY and MJQ designed the study and performed major experiments. MJQ and XWL supported the study. CL, LZ, and WC were in charge of collecting the information of patients. WC, WXY, and CL performed the bioinformatics analysis and statistics analysis. WXY and MJQ helped in writing the draft. This work has been presented in Research Square (https://doi.org/10.21203/rs.3.rs-293144/v1).

Corresponding author

Correspondence to Minjun Qi.

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The authors declared no conflicts of interest in this work.

Ethical Approval

The study was approved by the Regional Ethics Committee at the Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University.

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The consent was obtained from patients.

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Wu, X., Cao, W., Lu, C. et al. circ3323 Motivates Host Gene to Promote the Aggressiveness of Bladder Cancer. Biochem Genet 60, 2327–2345 (2022). https://doi.org/10.1007/s10528-022-10210-x

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  • DOI: https://doi.org/10.1007/s10528-022-10210-x

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