Abstract
Ovate Family Proteins (OFPs) belong to a plant-specific transcription factor family. They have been found to have significant roles in growth and development in Arabidopsis and tomato; however, little is known regarding their role in banana. Thus, a genome-wide study of OFP genes in banana was conducted for the first time in the present study. The results demonstrated that 49 OFP family members are unequally distributed across 11 chromosomes. Phylogenetic analysis grouped these genes into two subfamilies and eight subgroups, which was confirmed by the conserved motif and gene structure analysis. Furthermore, MaOFPs genes duplicates were found to have originated from whole-genome duplication (WGD). The expression patterns of the genes in the various tissues and at different fruit development and ripening stages in the BaXi Jiao (BX) and Feng Jiao (FJ), banana cultivars were elucidated using transcriptome analysis. Using co-expression network analysis, MaOFP1 was found to interact not only with MaMADS36 but also with hormone response proteins. These findings improve our understanding of the functions of MaOFPs genes in the control of plant hormone signal transduction pathways during banana growth and ripening, which should inform the genetic improvement of important agricultural characters.
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Data Availability
The identified OFP gene sequences of the DH-Pahang genome can be obtained from the banana genome database (https://banana-genome.cirad.fr/) (The accession numbers were listed in Supplementary Table S1).
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (NNSFC) (31872161), the National Natural Science Foundation of Hainan Province (2019CXTD412), the Central Public-interest Scientific Institution Basal Research Fund for Innovative Research Team Program of CATAS (1630052017018; 1630052019024) and the earmarked fund for Modern Agro-industry Technology Research System (CARS-31).
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JHL, ZQJ and BYXu conceived the study. JYW, CHJ and JBZ performed the experiments and carried out the analysis. JZ, HXM and BYXie designed the experiments and wrote the manuscript. All authors read and approved the final manuscript.
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Zhang, J., Miao, H., Xie, B. et al. Genomic and Transcriptional Analysis of Banana Ovate Family Proteins Reveals Their Relationship with Fruit Development and Ripening. Biochem Genet 58, 412–429 (2020). https://doi.org/10.1007/s10528-020-09951-4
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DOI: https://doi.org/10.1007/s10528-020-09951-4