Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro
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The inducible repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) eliminates O6-methylguanine adducts in DNA and protects the cells from damaging effects of alkylating agents. We have found that anti-MGMT antibodies recognize both the MGMT protein with a mol. weight ~ 24 kDa and a protein with a mol. weight ~ 48 kDa, which was named MARP (anti-methyltransferase antibody recognizable protein). A number of growth factors and cytokines were shown to regulate the expression of MGMT and MARP proteins. The ranges of concentrations of several growth factors and cytokines that caused increasing or decreasing protein amounts in human cell cultures were determined. The results of special biological experiments have allowed us to assume a possible role of MARP in the repair of alkyl adducts in human cells.
KeywordsCytokine DNA damage Repair enzyme MGMT Growth factor Antibody Human cells in vitro
We are very grateful to Professors Korneluyk A.I., Kuchuk M.V., Drs. Chernykh S.I., Potopalsky A.I., and Luchakovskaya Yu.S. for providing the preparations of cytokines and alkylating agent.
The study was supported by the National Academy of Sciences of Ukraine (No. 0115U000355) and the Czech Research Infrastructure for Systems Biology C4SYS (Project No. LM2015055).
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