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Effect of Zinc and Melatonin on Oxidative Stress and Serum Inhibin-B Levels in a Rat Testicular Torsion–Detorsion Model

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Abstract

The present study was aimed to examine the effects of 3-week zinc and melatonin administration on testicular tissue injury and serum Inhibin-B levels caused by unilateral testicular torsion–detorsion in rats. The study was performed on 60 Wistar Albino-type adult male rats. The animals were allocated to 6 groups in equal numbers. 1. Control; 2. Sham; 3. Ischemia–reperfusion; 4. Zinc + ischemia–reperfusion; 5. Melatonin + ischemia–reperfusion; 6. Zinc + melatonin + ischemia–reperfusion. Zinc and melatonin were administered before ischemia–reperfusion at doses of 5 and 3 mg/kg respectively, by intraperitoneal route for a period of 3 weeks. Testicular torsion–detorsion procedures consisted of ischemia for 1 h and then reperfusion for another hour of the left testis. Blood and testicular tissue samples were collected to analyze erythrocyte and tissue GSH and plasma and tissue MDA, Inhibin-B levels. The highest erythrocyte and testis GSH values were found in zinc, melatonin, and zinc + melatonin groups (p < 0.001). Torsion–detorsion group has significantly lower erythrocyte GSH levels and higher plasma MDA values (p < 0.001). Serum inhibin-B and spermatogenic activity levels in the torsion–detorsion group were also significantly lower than those in the other groups (p < 0.001). However, zinc-, melatonin-, and melatonin + zinc-supplemented groups have higher inhibin-B and spermatogenetic activity (p < 0.001). The results of the study show that zinc, melatonin, and melatonin + zinc administration partially restores the increased oxidative stress, as well as the reduced inhibin-B and spermatogenic activity levels in testes ischemia–reperfusion in rats. Suppressed inhibin-B levels in the testicular tissue may be a marker of oxidative stress.

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Acknowledgement

This study was supported by the Scientific Research Projects Coordinatorship of Selcuk University (SUBAPK; Project No. 12202010).

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Authors and Affiliations

  1. Department of Urology, Bagcilar Training and Research Hospital, Istanbul, Turkey

    Atilla Semercioz

  2. Faculty of Medicine, Department of Physiology, Selçuk University, 42075, Konya, Turkey

    Abdulkerim Kasim Baltaci & Rasim Mogulkoc

  3. Faculty of Meram Medicine, Department of Pathology, Necmettin Erbakan University, Konya, Turkey

    Mustafa Cihat Avunduk

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  1. Atilla Semercioz
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  2. Abdulkerim Kasim Baltaci
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Correspondence to Abdulkerim Kasim Baltaci.

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Conflict of interest

Authors declare no conflict of interest. The study protocol was approved by the Ethics Board of the Selcuk University’s School of Veterinary Medicine in (28 December 2011 and 2011/112).

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Semercioz, A., Baltaci, A.K., Mogulkoc, R. et al. Effect of Zinc and Melatonin on Oxidative Stress and Serum Inhibin-B Levels in a Rat Testicular Torsion–Detorsion Model. Biochem Genet 55, 395–409 (2017). https://doi.org/10.1007/s10528-017-9826-5

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