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Carbonic Anhydrase VI Gene Polymorphism rs2274327 Relationship Between Salivary Parameters and Dental-Oral Health Status in Children

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Abstract

The aim of this study was to research carbonic anhydrase (CA) VI one single-nucleotide polymorphism (SNP) and its potential association with dental-oral health status (dental caries, Plaque Index (PI) and Gingival Index (GI)) and salivary parameters (salivary buffering capacity, salivary flow rate (SFR)) in children. A total of 178 children were divided into two groups: non-carious (n = 70, 34 boys and 36 girls) and carious (n = 108, 47 boys and 61 girls). The clinical evaluations were performed according to the decayed, missing, and filled teeth (dmft/DMFT) index by a specialist. Clinical parameters including PI, GI, and simplified oral hygiene index (OHI-S) were recorded. Salivary pH (SpH) was measured using pH paper. Blood samples and unstimulated whole saliva were collected, and SFR was calculated. The CA VI rs2274327 polymorphism was determined by a LightSNiP assay on the realtime PCR system. The frequencies of rs2274327 were not significant between groups (p > 0.05). There was a positive correlation between OHI-S and SpH in the carious and non-carious groups (p < 0.05). There was no correlation among the SNPs’ frequencies and OHI-S, PI, GI, SFR, and SpH (p > 0.05). CA VI SNP (rs2274327) had no statistically significant association with OHI-S, PI, GI, SFR, and SpH in the children.

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Acknowledgments

The authors would like to thank the Ataturk University Scientific Research Projects Fund (2012/66–2012/375) for financial support.

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Authors and Affiliations

  1. Department of Pediatric Dentistry, Faculty of Dentistry, Ataturk University, Erzurum, Turkey

    Fatih Sengul, Munevver Kilic & Taskin Gurbuz

  2. Department of Medical Genetics, Medical Faculty, Ataturk University, Erzurum, Turkey

    Sener Tasdemir

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  1. Fatih Sengul
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  2. Munevver Kilic
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  3. Taskin Gurbuz
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  4. Sener Tasdemir
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Correspondence to Munevver Kilic.

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Sengul, F., Kilic, M., Gurbuz, T. et al. Carbonic Anhydrase VI Gene Polymorphism rs2274327 Relationship Between Salivary Parameters and Dental-Oral Health Status in Children. Biochem Genet 54, 467–475 (2016). https://doi.org/10.1007/s10528-016-9733-1

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