Skip to main content

Advertisement

Log in

RETRACTED ARTICLE: Inferring Phylogenetic Relationships of Indian Citron (Citrus medica L.) based on rbcL and matK Sequences of Chloroplast DNA

  • Original Article
  • Published:
Biochemical Genetics Aims and scope Submit manuscript

This article was retracted on 24 October 2019

This article has been updated

Abstract

Phylogenetic relationships of Indian Citron (Citrus medica L.) with other important Citrus species have been inferred through sequence analyses of rbcL and matK gene region of chloroplast DNA. The study was based on 23 accessions of Citrus genotypes representing 15 taxa of Indian Citrus, collected from wild, semi-wild, and domesticated stocks. The phylogeny was inferred using the maximum parsimony (MP) and neighbor-joining (NJ) methods. Both MP and NJ trees separated all the 23 accessions of Citrus into five distinct clusters. The chloroplast DNA (cpDNA) analysis based on rbcL and matK sequence data carried out in Indian taxa of Citrus was useful in differentiating all the true species and species/varieties of probable hybrid origin in distinct clusters or groups. Sequence analysis based on rbcL and matK gene provided unambiguous identification and disposition of true species like C. maxima, C. medica, C. reticulata, and related hybrids/cultivars. The separation of C. maxima, C. medica, and C. reticulata in distinct clusters or sub-clusters supports their distinctiveness as the basic species of edible Citrus. However, the cpDNA sequence analysis of rbcL and matK gene could not find any clear cut differentiation between subgenera Citrus and Papeda as proposed in Swingle’s system of classification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

Change history

  • 24 October 2019

    The Editor-in-Chief and the publisher have retracted this article [1] because of significant overlap with previously published articles [2, 3, 4, 5]. Ajit Uchoi, Surendra Kumar Malik, Ravish Chaudhary, Susheel Kumar, M.R. Rohini, Digvender Pal, and Sezai Ercisli disagree with the retraction. The publisher was not able to get in contact with Rekha Chaudhury, she did not respond to any correspondence about this retraction.

  • 24 October 2019

    The Editor-in-Chief and the publisher have retracted this article [1] because of significant overlap with previously published articles [2���5]. Ajit Uchoi, Surendra Kumar Malik, Ravish Chaudhary, Susheel Kumar, M.R. Rohini, Digvender Pal, and Sezai Ercisli disagree with the retraction. The publisher was not able to get in contact with Rekha Chaudhury, she did not respond to any correspondence about this retraction.

References

  • Altschul SF, Thomas LM, Alejandro AS, Jinghui Z, Zheng Z, Webb M, David JL (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25:3389–3402

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Anonymous (2015) Indian Horticulture Database 2014 (National Horticulture Board, Ministry of Agriculture, Gurgaon, Haryana), pp 1–286

  • Arau´jo EF, Queiroz LP, Machado MA (2003) What is citrus? Taxonomic implications from a study of cp-DNA evolution in the tribe Citreae (Rutaceae subfamily Aurantioideae). Org Div Evol 3:55–62

    Article  Google Scholar 

  • Bajpai PK, Warghat AR, Sharma RK, Yadav A, Thakur AK, Srivastava RB, Stobdan T (2014) Structure and genetic diversity of natural populations of Morus alba in the Trans-Himalayan Ladakh Region. Biochem Genet 52(3):137–152

    Article  CAS  PubMed  Google Scholar 

  • Barkley NA, Roose ML, Krueger RR, Federici CT (2006) Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor Appl Genet 112:1519–1531

    Article  CAS  PubMed  Google Scholar 

  • Barrett HC, Rhodes AM (1976) A numerical taxonomy study of affinity relationships in cultivated Citrus and its close relatives. Syst Bot 1:105–136

    Article  Google Scholar 

  • Bayer RJ, Mabberley DJ, Morton CM, Cathy H, Sharma IK, Pfeil BE, Rich S, Hitchcock R, Sykes S (2009) A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences. Am J Bot 96:668–685

    Article  CAS  PubMed  Google Scholar 

  • Bhattacharya SC, Dutta S (1956) Classification of Citrus fruits of Assam, Sc. Monogr. 20, ICAR, New Delhi, p. 110

  • Chase MW, Soltis DE, Olmstead RG, Morgan D, Les DH, Mishler BD, Duvall MR, Price RA, Hills HG, Qui YL (1993) Phylogenetics of seed plants: an analysis of nucleotide sequences from the plastid gene rbcL. Ann Missouri Bot Gard 80:528–580

    Article  Google Scholar 

  • Cheng YJ, Carmen M, Meng HJ, Guo WW, Tao NG, Deng XX (2005) A set of primers for analyzing chloroplast DNA diversity in Citrus and related genera. Tree Physiol 25:661–672

    Article  PubMed  Google Scholar 

  • Deng ZN, La-Malfa S, Xie XM, Xiong XG, Gentile A (2007) Identification and evaluation of chloroplast uni- and trinucleotide sequence repeats in citrus. Sci Hortic 111:186–192

    Article  CAS  Google Scholar 

  • FAO (2009) Food and Agricultural Organization of the United Nations. http://faostat.fao.org

  • Federici CT, Fang DQ, Scora RW, Roose ML (1998) Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theor Appl Genet 96:812–822

    Article  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  PubMed  Google Scholar 

  • Feng SG, Lu JJ, Gao L, Liu JJ, Wang HZ (2014) Molecular phylogeny analysis and species identification of Dendrobium (Orchidaceae) in China. Biochem Genet 52(3):127–136

    Article  CAS  PubMed  Google Scholar 

  • Frost HB, Soost RK (1968) Seed reproduction: development of gametes and embryos. In: Reuther W, Batchelor LD, Webber HB (eds) The Citrus industry, vol 2. California University Press, California, pp 290–324

    Google Scholar 

  • Gmitter FG, Hu X (1990) The possible role of Yunnan, China, in the origin of contemporary Citrus species (Rutaceae). Econ Bot 44:237–277

    Article  Google Scholar 

  • Groppo MG, Pirani JR, Salatino MLF, Blanco SR, Kallunki JA (2008) Phylogeny of Rutaceae based on two noncoding regions from cpDNA. Am J Bot 95:985–1005

    Article  CAS  PubMed  Google Scholar 

  • Gulsen O, Roose ML (2001) Chloroplast and nuclear genome analysis of parentage of lemons. J Am Soc Hortic Sci 126:210–215

    Article  CAS  Google Scholar 

  • Handa T, Ishizawa Y, Oogaki C (1986) Phylogenetic study of fraction I protein in the genus Citrus and its close related genera. Jp J Genet 61:15–42

    Article  Google Scholar 

  • Herrero R, Asins MJ, Carbonell EA, Navarro L (1996) Genetic diversity in the orange subfamily Aurantoideae. I. Interspecies and intragenus genetic variability. Theor Appl Genet 92:599–609

    Article  CAS  PubMed  Google Scholar 

  • Higgins D, Thompson J, Gibson T (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl Acids Res 22:4673–4680

    Article  PubMed  PubMed Central  Google Scholar 

  • Hilu KW, Liang H (1997) The matK gene: sequence variation and application in plant systematics. Am J Bot 84:830–839

    Article  CAS  PubMed  Google Scholar 

  • Hilu KW, Borsch T, Muller K, Soltis DE, Soltis PS (2003) Angiosperm phylogeny based on matK sequence information. Am J Bot 90:1758–1776

    Article  CAS  PubMed  Google Scholar 

  • Hirai M, Kozaki I (1981) Isozymes of Citrus leaves. Proc Int Soc Citric 1:10–13

    Google Scholar 

  • Hynniewta M, Malik SK, Rao SR (2014) Genetic diversity and phylogenetic analysis of Citrus (L) from north-east India as revealed by meiosis, and molecular analysis of internal transcribed spacer region of rDNA. Meta Gene 2:237–251

    Article  PubMed  PubMed Central  Google Scholar 

  • Jena SN, Kumar S, Nair NK (2009) Molecular phylogeny in Indian Citrus L. (Rutaceae) inferred through PCR-RFLP and trnL-trnF sequence data of chloroplast DNA. Sci Hortic 119:403–416

    Article  CAS  Google Scholar 

  • Jung YH, Kwon HM, Kang SH, Kang JH, Kim SC (2005) Investigation of the phylogenetic relationships within the genus Citrus (Rutaceae) and related species in Korea using plastid trnL-trnF sequences. Sci Hortic 104:179–188

    Article  CAS  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Nair KN, Jena SN (2012) Molecular differentiation in Indian Citrus L. (Rutaceae) inferred from nrDNA ITS sequence analysis. Genet Resour Crop Evol 60:59–75. doi:10.1007/s10722-012-9814-x

    Article  CAS  Google Scholar 

  • Kyndt T, Dung TN, Goetghebeur P, Toan HT, Gheysen G (2010) Analysis of ITS of the rDNA to infer phylogenetic relationship among Vietnamese Citrus accessions. Genet Resour Crop Evol 57:183–192

    Article  CAS  Google Scholar 

  • Li YZ, Cheng YJ, Tao NG, Deng XX (2007) Phylogenetic analysis of mandarin landraces, wild mandarins, and related species in China using nuclear LEAFY second intron and plastid trnL-trnF sequence. J Am Soc Hortic Sci 132:796–806

    Article  CAS  Google Scholar 

  • Liang G, Xiong G, Guo Q, He Q, Li X (2007) AFLP analysis and the taxonomy of Citrus. Acta Hortic 760:137–142

    Article  CAS  Google Scholar 

  • Lu ZH, Zhou ZQ, Xie RJ (2011) Molecular phylogeny of the ‘‘true citrus fruit trees’’ group (Aurantioideae, Rutaceae) as inferred from chloroplast DNA sequence. Agric Sci China 10:49–57

    Article  CAS  Google Scholar 

  • Luro F, Laigret F, Bove JM, Ollitrault P (1995) DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus. Hortic Sci 30:1063–1067

    CAS  Google Scholar 

  • Mabberley DJ (2004) Citrus (Rutaceae): a review of recent advances in etymology, systematics and medical applications. Blumea 49:481–498

    Article  Google Scholar 

  • Malik MN, Scora RW, Soost RK (1974) Studies on the origin of lemon. Hilgardia 42:361–382

    Article  CAS  Google Scholar 

  • Malik SK, Kumar S, Singh IP, Dhariwal OP, Chaudhury R (2013) Socio-economic importance, domestication trends and in situ conservation of wild Citrus species of Northeast India. Genet Resour Crop Evol. doi:10.1007/s10722-012-9948-x

    Article  Google Scholar 

  • Mlcek J, Valsikova M, Druzbikova H, Ryant P, Jurikova T, Sochor J, Borkovcova M (2015) The antioxidant capacity and macroelement content of several onion cultivars. Turk J Agri For 39:999–1004

    Article  CAS  Google Scholar 

  • Moore GA (2001) Oranges and lemons: clues to the taxonomy of Citrus from molecular markers. Trends Genet 17:536–540

    Article  CAS  PubMed  Google Scholar 

  • Morton CM, Grant M, Blackmore S (2003) Phylogenetic relationships of the Aurantioideae inferred from chloroplast DNA sequence data. Am J Bot 90:1463–1469

    Article  CAS  PubMed  Google Scholar 

  • Nair KN, Nayar MP (1997) Rutaceae. In: Hajra PK, Nair VJ, Daniel P (eds) Flora of India, vol IV. Botanical Survey of India, Calcutta, pp 229–407

    Google Scholar 

  • Nicolosi E, Deng ZN, Gentile A, La Malfa S, Continella G, Tribulato E (2000) Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet 100:1155–1166

    Article  CAS  Google Scholar 

  • Olmstead RG, Palmer JD (1994) Chloroplast DNA systematics: a review of methods and data analysis. Am J Bot 81:1205–1224

    Article  CAS  Google Scholar 

  • Rogstad SH (1993) Saturated NaCl-CTAB solution as a means of field preservation leaves for DNA analysis. Taxon 41:701–708

    Article  Google Scholar 

  • Ruttanaprasert R, Banterng P, Jogloy S, Vorasoot N, Kesmala T, Kanwar RS, Holbrook CC, Patanothai A (2014) Genotypic variability for tuber yield, biomass, and drought tolerance in Jerusalem artichoke germplasm. Turk J Agri For 38:570–580

    Article  Google Scholar 

  • Salvo G, Ho SY, Rosenbaum G, Ree R, Conti E (2010) Tracing the temporal and spatial origins of island endemics in the Mediterranean region: a case study from the citrus family (Ruta L., Rutaceae). Syst Biol 59:705–722

    Article  PubMed  Google Scholar 

  • Scora RW (1975) On the history and origin of citrus. Bull Torrey Bot Club 102:369–375

    Article  Google Scholar 

  • Sharma BD, Hore DK, Gupta SG (2004) Genetic resources of Citrus of north-eastern India and their potential use. Genet Resour Crop Evol 51:411–418

    Article  Google Scholar 

  • Small RL, Lickey EB, Shaw J, Hauk WD (2005) Amplification of non-coding chloroplast DNA for phylogenetic studies in lycophytes and monilophytes with comparative example of relative phylogenetic utility from ophioglossaceae. Mol Phylo Evol 36(507):522

    Google Scholar 

  • Swingle WT (1943) The botany of Citrus and its wild relatives. In: Webber HJ, Batchelor DL (eds) The citrus industry, vol 1. University of California, Berkeley, pp 128–474

    Google Scholar 

  • Swingle WT, Reece PC (1967) The botany of citrus and its wild relatives. In: Reuther W, Webber HJ, Batchelor LD (eds) The citrus industry, vol 1. University of California Press, Berkeley, CA, USA, pp 389–390

    Google Scholar 

  • Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenetics by using the NJ methods. Proc Nat Acad Sci (USA) 101:11030–11035

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M et al (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony method. Mol Biol Evol 28:2731–2739

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tanaka T (1928) On certain new species of Citrus. Studia Citrol 2:155–164 Japanese with English, Latin resume

    Google Scholar 

  • Tanaka T (1977) Fundamental discussion of citrus classification. Studia Citrologia 14:1–6

    Google Scholar 

  • Torres AM, Soost RK, Diedenhofen U (1978) Leaf isozymes as genetic markers in Citrus. Am J Bot 65:869–881

    Article  Google Scholar 

  • Tshering P, Anai T, Nagano Y, Matsumoto R, Yamamoto M (2010) Phylogenetic relationships of Citrus and its relatives based on rbcL gene sequences. Tree Genet Genomes 6:931–939

    Article  Google Scholar 

  • Tshering P, Yamamoto M, Ide MUM, Matsumoto N, Matsumoto R, Nagano Y (2013) Phylogenetic relationships of citrus and its relatives based on matK gene sequences. PLoS ONE 8(4):e62574

    Article  CAS  Google Scholar 

  • Uzun A, Yesiloglu T, Aka-Kacar Y, Tuzcu O, Gulsen O (2009) Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Sci Hort 121:306–312

    Article  CAS  Google Scholar 

  • Wali S, Munir F, Mahmood T (2013) Phylogenetic studies of selected Citrus species based on chloroplast gene, rps14. Int J Agric Biol 15:357–361

    CAS  Google Scholar 

  • Webber HJ (1943) Cultivated varieties of Citrus. In: Webber HJ, Batchelor DL (eds) The Citrus Industry, vol 1. University of California, Berkeley, pp 475–668

    Google Scholar 

  • Zhu LW (1988) Numerical taxonomy of Citrus species in China (in Chinese with English abstract). Acta Phytotaxon Sin 26:353–361

    Google Scholar 

Download references

Acknowledgments

The authors are grateful to Director, NBPGR and Head, Exploration and Collection Division, NBPGR, New Delhi for encouragement and support. The authors thank the officers and staff of forest departments and ICAR institutes/centres in Northeast India for assistance and support during exploration and survey trips. The authors are also thankful to Post Graduate School, IARI and University Grant Commission for awarding Doctorate Fellowship.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Surendra Kumar Malik.

Additional information

The Editor-in-Chief and the publisher have retracted this article because of significant overlap with previously published articles. Ajit Uchoi, Surendra Kumar Malik, Ravish Chaudhary, Susheel Kumar, M.R. Rohini, Digvender Pal, and Sezai Ercisli disagree with the retraction. The publisher was not able to get in contact with Rekha Chaudhury. She did not respond to any correspondence about this retraction.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Uchoi, A., Malik, S.K., Choudhary, R. et al. RETRACTED ARTICLE: Inferring Phylogenetic Relationships of Indian Citron (Citrus medica L.) based on rbcL and matK Sequences of Chloroplast DNA. Biochem Genet 54, 249–269 (2016). https://doi.org/10.1007/s10528-016-9716-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-016-9716-2

Keywords