Isolation and Characterization of 14 Polymorphic Microsatellite Loci in the Big-Headed Turtle (Platysternon megacephalum)
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The big-headed turtle (Platysternon megacephalum) is critically endangered because of overharvesting, illegal trade, and habitat destruction. Assessment of genetic variability in existing populations becomes very important to the taxonomy and conservation of this species. Here we describe 14 microsatellite loci isolated from an enriched genomic library of the big-headed turtle, and the polymorphisms of these loci were assessed in 28 individuals from Huizhou, Heyuan, Zhaoqing, and Shaoguan of Guangdong, China. The range of polymorphism information content is 0.305–0.738, and no evidence of significant linkage disequilibrium was found among any pairs of loci. These 14 new polymorphic microsatellite loci can be used in population genetics, taxonomy, phylogeography, behavior ecology, and conservation efforts of Platysternon megacephalum.
KeywordsBig-headed turtle Platysternon megacephalum Microsatellite loci Isolation Conservation
We thank Mr. Haijing Wang, Mr. Dongsheng Pan, Mr. Xiangjing Zhong, Mr. Taojun, and Mr. Chenyu for their help during the collection of tissue samples of turtles. This study was supported financially by the Zhujiang Scientific and Technological New Star Program (2011J2200025), the National Natural Science Foundation of China (No. 31071935), the Scientific and Technological Innovation and Guiding Program of the Guangdong Provincial Academy of Science (No. cx2011), the Guangdong Natural Science Foundation of China (No. S2011010000514), and the Scientific and Technological Program of Huizhou, Guangdong, China (No. 2008P09).
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