Biochemical Genetics

, Volume 52, Issue 3–4, pp 203–208 | Cite as

Isolation and Characterization of 14 Polymorphic Microsatellite Loci in the Big-Headed Turtle (Platysternon megacephalum)

  • Liushuai Hua
  • Fumin Wang
  • Shiping Gong
  • Yan Ge
  • Guoling Chen
  • Jinping Chen
Article

Abstract

The big-headed turtle (Platysternon megacephalum) is critically endangered because of overharvesting, illegal trade, and habitat destruction. Assessment of genetic variability in existing populations becomes very important to the taxonomy and conservation of this species. Here we describe 14 microsatellite loci isolated from an enriched genomic library of the big-headed turtle, and the polymorphisms of these loci were assessed in 28 individuals from Huizhou, Heyuan, Zhaoqing, and Shaoguan of Guangdong, China. The range of polymorphism information content is 0.305–0.738, and no evidence of significant linkage disequilibrium was found among any pairs of loci. These 14 new polymorphic microsatellite loci can be used in population genetics, taxonomy, phylogeography, behavior ecology, and conservation efforts of Platysternon megacephalum.

Keywords

Big-headed turtle Platysternon megacephalum Microsatellite loci Isolation Conservation 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Liushuai Hua
    • 1
  • Fumin Wang
    • 2
  • Shiping Gong
    • 1
  • Yan Ge
    • 1
  • Guoling Chen
    • 1
  • Jinping Chen
    • 1
  1. 1.Guangdong Provincial Public Laboratory for Wild Animal Conservation and ManagementGuangdong Entomological Institute (South China Institute of Endangered Animals)GuangzhouChina
  2. 2.Guangdong Provincial Wildlife Rescue CenterGuangzhouChina

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