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Effect of Actin Polymerization Inhibitor During Oocyte Maturation on Parthenogenetic Embryo Development and Ploidy in Capra hircus

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Abstract

This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species.

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Acknowledgments

The authors are thankful to the Director, IVRI, Izatnagar, for providing all necessary facilities to conduct this experiment.

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Authors and Affiliations

  1. Reproductive Physiology and Embryo Transfer Technology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India

    R. Ranjan, R. K. Singh, T. Yasotha, Manish Kumar, Kuldeep Kumar, Renu Singh, B. C. Das & Sadhan Bag

  2. College of Veterinary Sciences and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat, India

    Gopal Puri

  3. Biochemistry Division, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India

    Sanjeev Bhure

  4. Department of Biotechnology, National Dairy Research Institute, Karnal, India

    D. Malakar

  5. Poultry Housing and Management, Central Avian Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India

    S. K. Bhanja

  6. Endocrinology Laboratory, Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India

    M. Sarkar

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  1. R. Ranjan
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  2. R. K. Singh
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  3. T. Yasotha
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  4. Manish Kumar
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  5. Gopal Puri
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  6. Kuldeep Kumar
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  7. Renu Singh
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  8. Sanjeev Bhure
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  9. D. Malakar
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  10. S. K. Bhanja
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  11. M. Sarkar
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  12. B. C. Das
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  13. Sadhan Bag
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Corresponding author

Correspondence to Sadhan Bag.

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Ranjan, R., Singh, R.K., Yasotha, T. et al. Effect of Actin Polymerization Inhibitor During Oocyte Maturation on Parthenogenetic Embryo Development and Ploidy in Capra hircus . Biochem Genet 51, 944–953 (2013). https://doi.org/10.1007/s10528-013-9619-4

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  • DOI: https://doi.org/10.1007/s10528-013-9619-4

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