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Genetic Predisposition for Development of Nephropathy in Type 2 Diabetes Mellitus

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Abstract

The aim of the study was to explore the association of the angiotensin-converting enzyme (ACE) gene I/D polymorphism and the methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism with development of diabetic nephropathy in type 2 diabetes mellitus. Three groups were recruited during 2007–2011: 232 normal controls, 185 type 2 diabetics without nephropathy, and 407 type 2 diabetics with nephropathy. The ACE I/D and MTHFR C677T polymorphisms were examined using PCR and PCR-RFLP methods. We found no significant association of the ACE I/D polymorphism with diabetic nephropathy in genotype, allele, dominant, and recessive models. We observed a significant association of MTHFR C677T with development of diabetic nephropathy in type 2 diabetics. The MTHFR C677T polymorphism plays a significant role in predisposition of renal insufficiency in diabetic patients.

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Acknowledgments

The authors are thankful to the Indian Council of Medical Research (ICMR), New Delhi, for financial assistance and to the Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGI), Lucknow, for providing the infrastructure. The support of the patients, consultants, and the staff of the nephrology ward and outpatient door of the Institute for participating in the study is gratefully acknowledged. The authors are thankful to Dr. Priti Ojha for her technical support. RK is thankful to CSIR, New Delhi, for his fellowship.

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  1. Department of Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, 226014, Uttar Pradesh, India

    Ravindra Kumar & Sarita Agarwal

  2. Department of Nephrology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, 226014, Uttar Pradesh, India

    Raj Kumar Sharma

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  1. Ravindra Kumar
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Correspondence to Sarita Agarwal.

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Kumar, R., Sharma, R.K. & Agarwal, S. Genetic Predisposition for Development of Nephropathy in Type 2 Diabetes Mellitus. Biochem Genet 51, 865–875 (2013). https://doi.org/10.1007/s10528-013-9613-x

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