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Genetic Diversity of Kenaf (Hibiscus cannabinus) Evaluated by Inter-Simple Sequence Repeat (ISSR)

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Abstract

Understanding genetic diversity is very useful for scientific utilization for breeding. In this study, we estimated the genetic distances in a panel of 84 kenaf accessions collected from 26 countries and regions using ISSR markers. The results of UPGMA analysis showed that kenaf germplasm had abundant genetic variation, with genetic dissimilarity coefficients ranging from 0.01 to 0.62. The in-group dissimilarity coefficient (0.29) was observed in 84 kenaf accessions, and all the accessions could be divided into three groups: cultivars (L1–1), relatively wild species (L1–2 and L1–3), and wild species (the others). Further in-group analysis in group L1–1 (0.19) revealed that the kenaf cultivars could be divided into five subgroups with distinct regional characteristics. It is imperative that genes be exchanged among all kinds of tested varieties from different origins. The results provide a useful basis for kenaf germplasm research and breeding.

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References

  • Chen A, Li D, Tan H (2005) Analysis on kenaf cultivar genealogy and breeding tactics in China. Plant Fiber Prod 27(4):176–180

    Google Scholar 

  • Chen M, Wei C, Qi J, Chen X, Su J, Li A, Tao A, Wu W (2011) Genetic linkage map construction for kenaf using SRAP, ISSR and RAPD markers. Plant Breed 130:79–687

    Google Scholar 

  • Cheng Z, Lu B, Sameshima K, Chen J (2002) Comparative studies of genetic diversity in kenaf (Hibiscus cannabinus L.) varieties based on analysis of agronomic and RAPD data. Hereditas 136:231–239

    Article  PubMed  Google Scholar 

  • Cheng Z, Yang X, Lu B, Jiao D, Chen J (2003) Genetic diversity and molecular identification of kenaf germplasm II, Diversity and genetic relationships of kenaf germplasm assessed by AFLP analysis. Plant Fiber Prod 25:162–167

    Google Scholar 

  • Deng L, Su J, Huang P, Li A (1991) Morphological and taxonomic studies of kenaf germplasm. China’s Fiber Crops 4:16–20

    Google Scholar 

  • Guo A, Zhou P, Su J (2002) Random amplified polymorphic DNA (RAPD) analyses among Hibiscus cannabinus and related species. J Trop Subtrop Bot 10:306–312

    CAS  Google Scholar 

  • Li A, Deng L, Su J (1992) The preliminary identification of wild kenaf and relative species in East-Africa. J Anhui Agricul Sci 20:325–327

    Google Scholar 

  • Li D, He X, Liu G, Huang B (2011) Genetic diversity and phylogenetic relationship of Tadehagi in southwest China evaluated by inter-simple sequence repeat (ISSR). Genet Resour Crop Evol 58:679–688

    Article  Google Scholar 

  • Murray M, Thompson W (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Qi J, Li W, Wu W (1997) Studies on the origin and evolution of jute. Acta Agron Sin 23:677–682

    Google Scholar 

  • Qi J, Li W, Wu W, Lin L, Lin P, Lu Q, Zheng Y (1999) Theory and practice of kenaf germplasm innovation. China’s Fiber Crops 21:33–44

    Google Scholar 

  • Qi J, Fang P, Xu J, Lin L, Wu J (2007) High efficient utilization of jute/kenaf biomass and construction of industrial zone for territorial development. Plant Fiber Sci China 29:57–63

    Google Scholar 

  • Rohlf F (2000) NTsys-pc: numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Publishing, Setauket

    Google Scholar 

  • Su J (2002) Strategies for protection and sustainable utilization of genetic diversity of bast fiber crops in China. J Plant Genet Resour 3:41–46

    Google Scholar 

  • Su J, Deng L (1996) Study on the morphology and taxonomy of Hibiscus L. China’s Fiber Crops 18:18–20

    Google Scholar 

  • Su J, Gong Y, Guan F, Guang F, Lu Y, Li Y, Dai Z (2003) The collection, conservation, regeneration and utilization of bast fiber crop germplasms. Plant Fiber Sci China 25:4–9

    Google Scholar 

  • Xiong H (1989) Report of the germplasm collecting for jute and kenaf in Tanzania. China’s Fiber Crops 3:18–22

    Google Scholar 

  • Xu J, Qi J, Fang P, Li A, Lin L, Wu J, Tao A (2007) Optimized CTAB protocol for extracting genomic DNA from kenaf and improved PCR amplifications of ISSR and SRAP. Plant Fiber Sci China 29:179–183

    CAS  Google Scholar 

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176–183

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This project was sponsored by funds from the National Natural Science Foundation of China (30571188); the National Agri-Industry Technology Research System for Crops of Bast and Leaf Fiber, China (nycytx-19-E05); and the Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian (2010N2002).

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Authors and Affiliations

  1. Key Laboratory of Ministry for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 35002, China

    Liwu Zhang

  2. Anhui Seed Management Station, Hefei, 230051, China

    Aiqing Li

  3. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 35002, China

    Xiaofei Wang, Jiantang Xu, Guangqing Zhang & Jianmin Qi

  4. Bast Fiber Institute of the Chinese Academy of Agricultural Sciences, Changsha, 410006, China

    Jianguang Su

  5. Hunan Agriculture University, Changsha, 410128, China

    Xiaofei Wang & Chunyun Guan

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  1. Liwu Zhang
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  2. Aiqing Li
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  3. Xiaofei Wang
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  4. Jiantang Xu
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  5. Guangqing Zhang
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  6. Jianguang Su
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  7. Jianmin Qi
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  8. Chunyun Guan
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Corresponding author

Correspondence to Jianmin Qi.

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Liwu Zhang and Aiqing Li contributed equally to this work.

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Zhang, L., Li, A., Wang, X. et al. Genetic Diversity of Kenaf (Hibiscus cannabinus) Evaluated by Inter-Simple Sequence Repeat (ISSR). Biochem Genet 51, 800–810 (2013). https://doi.org/10.1007/s10528-013-9608-7

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  • DOI: https://doi.org/10.1007/s10528-013-9608-7

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