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Population Genetic Study of Fagopyrum tataricum from Western Himalaya Using ISSR Markers

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Abstract

Inter-simple sequence repeat (ISSR) markers were used to analyze genetic diversity and relatedness of 15 germplasms of Fagopyrum tataricum. Samples representing 75 individuals were collected from a range of altitudes in the Western Himalaya. The 13 ISSR primers revealed 98.1% polymorphism among populations, whereas average polymorphism was extremely low (2.18%) within populations. The coefficient of population differentiation was 0.9750, with limited gene flow (N m) of 0.0128. The average PIC value of the ISSR markers was high (0.812), with a marker ratio of 0.65 and marker index of 6.66. The genetic diversity of F. tataricum significantly correlated with altitude and gene diversity, Shannon’s index, and the percentage of polymorphic bands. The genetic diversity among populations showed broad genetic base and provided a developmental strategy for crop improvement.

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Acknowledgments

The authors express their gratitude to the Uttaranchal Council of Science and Technology and the Department of Science and Technology for providing support for all the experimental equipment. Thanks are also due to Dr. Vikas S. Jadon and Shashi Ranjan for collection of samples.

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Authors and Affiliations

  1. Molecular Biology and Nanotechnology Laboratory, Center for Biotechnology, University of Allahabad, Allahabad, Uttar Pradesh, India

    Garima Kishore & Anjana Pandey

  2. Department of Biotechnology, SBS PG Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India

    Garima Kishore & Sanjay Gupta

  3. Uttarakhand Council of Science and Technology, Dehradun, Uttarakhand, India

    Rajendra Dobhal

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  1. Garima Kishore
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  2. Anjana Pandey
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  3. Rajendra Dobhal
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  4. Sanjay Gupta
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Correspondence to Garima Kishore.

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Kishore, G., Pandey, A., Dobhal, R. et al. Population Genetic Study of Fagopyrum tataricum from Western Himalaya Using ISSR Markers. Biochem Genet 51, 750–765 (2013). https://doi.org/10.1007/s10528-013-9604-y

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  • DOI: https://doi.org/10.1007/s10528-013-9604-y

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