Abstract
This study investigated the role of −1607 (1G/2G) (rs1799750) polymorphism of the MMP-1 gene in chronic pancreatitis. We genotyped 100 patients with chronic pancreatitis and 100 control subjects using tetra-primer ARMS-PCR followed by agarose gel electrophoresis. Serum levels of MMP-1 were determined by Elisa. Statistical analysis was applied to test the significance of the results. The genotypic and allelic distribution varied significantly between the disease group and the control subjects [OD = 1.981 (1.236–3.181), p = 0.004]. MMP-1 levels were higher in subjects homozygous for the 2G allele than in subjects with the 1G allele. The present study revealed a significant association of the MMP-1 −1607 1G/2G (rs1799750) gene promoter polymorphism with chronic pancreatitis, and it can be considered a biological marker in the etiology of chronic pancreatitis.
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The authors acknowledge financial support from the University Grants Commission (UGC), New Delhi. The authors are also thankful to the Department of Biotechnology, New Delhi, for providing laboratory facilities.
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Sri Manjari, K., Nallari, P., Balakrishna, N. et al. Influence of Matrix Metalloproteinase-1 Gene −1607 (1G/2G) (rs1799750) Promoter Polymorphism on Circulating Levels of MMP-1 in Chronic Pancreatitis. Biochem Genet 51, 644–654 (2013). https://doi.org/10.1007/s10528-013-9594-9
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DOI: https://doi.org/10.1007/s10528-013-9594-9