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Influence of Matrix Metalloproteinase-1 Gene −1607 (1G/2G) (rs1799750) Promoter Polymorphism on Circulating Levels of MMP-1 in Chronic Pancreatitis

Biochemical Genetics volume 51, pages 644–654 (2013)Cite this article

Abstract

This study investigated the role of −1607 (1G/2G) (rs1799750) polymorphism of the MMP-1 gene in chronic pancreatitis. We genotyped 100 patients with chronic pancreatitis and 100 control subjects using tetra-primer ARMS-PCR followed by agarose gel electrophoresis. Serum levels of MMP-1 were determined by Elisa. Statistical analysis was applied to test the significance of the results. The genotypic and allelic distribution varied significantly between the disease group and the control subjects [OD = 1.981 (1.236–3.181), p = 0.004]. MMP-1 levels were higher in subjects homozygous for the 2G allele than in subjects with the 1G allele. The present study revealed a significant association of the MMP-1 −1607 1G/2G (rs1799750) gene promoter polymorphism with chronic pancreatitis, and it can be considered a biological marker in the etiology of chronic pancreatitis.

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Acknowledgments

The authors acknowledge financial support from the University Grants Commission (UGC), New Delhi. The authors are also thankful to the Department of Biotechnology, New Delhi, for providing laboratory facilities.

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Affiliations

  1. Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016, India

    K. Sri Manjari, A. Jyothy & A. Venkateshwari

  2. Department of Gastroenterology, Gandhi General Hospital, Secunderabad, India

    A. Vidyasagar

  3. Department of Gastroenterology, Osmania General Hospital, Hyderabad, India

    B. Prabhakar

  4. Department of Genetics, Osmania University, Hyderabad, India

    Pratibha Nallari

  5. National Institute of Nutrition, Hyderabad, India

    N. Balakrishna

Authors
  1. K. Sri Manjari
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  2. Pratibha Nallari
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  3. N. Balakrishna
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  4. A. Vidyasagar
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  5. B. Prabhakar
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  6. A. Jyothy
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  7. A. Venkateshwari
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Correspondence to A. Venkateshwari.

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Sri Manjari, K., Nallari, P., Balakrishna, N. et al. Influence of Matrix Metalloproteinase-1 Gene −1607 (1G/2G) (rs1799750) Promoter Polymorphism on Circulating Levels of MMP-1 in Chronic Pancreatitis. Biochem Genet 51, 644–654 (2013). https://doi.org/10.1007/s10528-013-9594-9

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  • DOI: https://doi.org/10.1007/s10528-013-9594-9

Keywords

  • Matrix metalloproteinase
  • Genotypes
  • Pancreatic fibrosis
  • Pancreatic stellate cells