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GT Microsatellite Repeats in the Heme Oxygenase-1 Gene Promoter Associated with Abdominal Aortic Aneurysm in Croatian Patients

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Abstract

Abdominal aortic aneurysm (AAA) is a complex genetic disorder caused by the interplay of genetic and environmental risk factors. The number of (GT) n repeats in the heme oxygenase-1 (HO-1) gene promoter modulates transcription of this enzyme, which might have anti-inflammatory, antioxidant, antiapoptotic, and antiproliferative effect. The distribution of alleles and genotypes in Croatian individuals genotyped for the (GT) n HO-1 polymorphism was similar to that in other European populations. Frequency of the short (S) alleles (GT < 25) was higher in AAA patients (41.9%) than in non-AAA individuals (28.2%, p = 0.0026) because there were more SL heterozygotes among the AAA patients. The SL genotype appeared to increase the risk for AAA, but the increase was not statistically significant after adjustment for age, sex, smoking, hypertension, and hyperlipidemia (OR = 1.53, 95% CI 0.90–3.09, p = 0.062). These findings contradict those of the only other study performed so far on the association of (GT) n HO-1 polymorphism and AAA.

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Acknowledgments

This work was supported by grant 006-006-1194-1218 from the Ministry of Science, Education and Sports of the Republic of Croatia.

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Authors and Affiliations

  1. Division of Vascular Surgery, Clinic of Surgery, University Hospital Center Zagreb, Zagreb, Croatia

    Andrea Crkvenac Gregorek

  2. Division of Genetics, Clinic of Pediatrics University Hospital Center Zagreb, Zagreb, Croatia

    Kristina Crkvenac Gornik

  3. Galapagos Research Center Ltd, Prilaz baruna Filipovica, Zagreb, Croatia

    Darija Stupin Polancec

  4. Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovacica 1, 10000, Zagreb, Croatia

    Sanja Dabelic

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  1. Andrea Crkvenac Gregorek
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  2. Kristina Crkvenac Gornik
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Correspondence to Sanja Dabelic.

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Gregorek, A.C., Gornik, K.C., Polancec, D.S. et al. GT Microsatellite Repeats in the Heme Oxygenase-1 Gene Promoter Associated with Abdominal Aortic Aneurysm in Croatian Patients. Biochem Genet 51, 482–492 (2013). https://doi.org/10.1007/s10528-013-9579-8

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  • DOI: https://doi.org/10.1007/s10528-013-9579-8

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