Abstract
The high osmolarity glycerol (HOG) signaling pathway is crucial for yeast to cope with high osmolarity. Here, we showed that Saccharomyces cerevisiae rice wine isolates exhibited higher tolerance to osmotic stress, which was associated with the evolution of HOG pathway genes. Phylogenetic analysis of HOG genes revealed that Chinese rice wine strains were closely related to sake strains, indicating a common origin of rice wine strains. The DNA sequence diversity analysis showed that higher levels of polymorphism tended to accumulate on the osmosensor genes (MSB2 and SLN1), suggesting that most changes in a signaling transduction pathway were concentrated in the receptors. Moreover, the rapid evolution of osmosensors (Sln1/Msb2) and transcription factor (Msn4) might experience positive selection. Our results imply that the evolution of HOG pathway genes in S. cerevisiae rice wine strains is associated with their adaptation to high osmotic environments.
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Acknowledgments
We thank Dr. Xiaoqin Chi (Zhejiang University) for her technical help during the course of the project. This work was supported by grants from the National Natural Science Foundation of China (No. 31101339) to Y. Li and the Zhejiang Provincial Natural Science Foundation (No. Y3100609) to X. Liang.
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Li, Y., Chen, W., Shi, Y. et al. Molecular Cloning and Evolutionary Analysis of the HOG-Signaling Pathway Genes from Saccharomyces cerevisiae Rice Wine Isolates. Biochem Genet 51, 296–305 (2013). https://doi.org/10.1007/s10528-012-9563-8
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DOI: https://doi.org/10.1007/s10528-012-9563-8