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Correlation of Peroxisome Proliferator-Activated Receptor (PPAR-γ) mRNA Expression with Pro12Ala Polymorphism in Obesity

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Abstract

Our study aimed to analyze whether the expression of PPARγ mRNA in subcutaneous adipocyte tissue correlates with Pro12Ala PPARγ2 polymorphism in the obesity context. We found that mRNA expression of PPARγ in subcutaneous adipose tissue was greater in obese subjects (P < 0.05) than in the nonobese control group. Concurrently, genotyping of the Pro12Ala polymorphism showed that obese subjects possess a significantly higher frequency of the Pro/Pro genotype than nonobese controls (90.5 vs 79.5%; P = 0.03), suggesting that this genotype is involved in an increased risk of obesity in the Tunisian population. Taken together, our results demonstrate that the Pro12 allele is accompanied by an overexpression of PPARγ mRNA in subcutaneous adipocyte tissue, suggesting that the PPARγ Pro12Ala variant may contribute to the observed variability in PPARγ mRNA expression and consequently in body mass index and insulin sensitivity in the general population.

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Authors and Affiliations

  1. Laboratory of Genetics, Immunology and Human Pathologies, Faculty of Sciences of Tunis, University of El Manar 1, 2092, Tunis, Tunisia

    Rym Berhouma, Soumaya Kouidhi, Myriam Ammar, Hajer Ennafaa & Amel Benammar-Elgaaied

  2. Hospital of Charles Nicolle, Tunis, Tunisia

    Hafawa Abid

Authors
  1. Rym Berhouma
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  2. Soumaya Kouidhi
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  3. Myriam Ammar
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  4. Hafawa Abid
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  5. Hajer Ennafaa
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  6. Amel Benammar-Elgaaied
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Correspondence to Rym Berhouma.

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R. Berhouma and S. Kouidhi contributed equally to this study.

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Berhouma, R., Kouidhi, S., Ammar, M. et al. Correlation of Peroxisome Proliferator-Activated Receptor (PPAR-γ) mRNA Expression with Pro12Ala Polymorphism in Obesity. Biochem Genet 51, 256–263 (2013). https://doi.org/10.1007/s10528-012-9560-y

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  • DOI: https://doi.org/10.1007/s10528-012-9560-y

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