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Sensitivity of Translation Initiation Factor eIF1 as a Molecular Target of Salt Toxicity to Sodic-Alkaline Stress in the Halophytic Grass Leymus chinensis

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Abstract

Eukaryotic translation initiation factors (eIFs) have been shown to be critical in the initiation of protein synthesis. Here, we report the cloning and characterization of a novel gene, LceIF1, from a potentially interesting forage grass, Leymus chinensis (Trin.). The expression results show that LceIF1 is expressed in most organisms under normal conditions, but the transcription patterns differ under sodic-saline and sodic-alkaline stresses. Sodic-saline stress induced a persistent decrease, and sodic-alkaline stress induced overexpression of LceIF1. Potassic-saline and alkaline stresses did not cause any changes in expression of eIF1. These results indicate that not only pH but also Na+ concentration affects overtranscription of LceIF1. The eIF1 transgenic lines showed relatively high eIF1 expression, resulting in potentially higher stress resistance. Combined with eIF1 transcription in transgenic lines, LceIF1 as a molecular target of salt toxicity is believed to help enhance salt tolerance.

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Acknowledgments

This work was supported by the Nutraceutical Bio Brain Korea 21 Project Group.

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Correspondence to Soon-Kwan Hong.

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Sun, YL., Hong, SK. Sensitivity of Translation Initiation Factor eIF1 as a Molecular Target of Salt Toxicity to Sodic-Alkaline Stress in the Halophytic Grass Leymus chinensis . Biochem Genet 51, 101–118 (2013). https://doi.org/10.1007/s10528-012-9546-9

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  • DOI: https://doi.org/10.1007/s10528-012-9546-9

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