Abstract
Eukaryotic translation initiation factors (eIFs) have been shown to be critical in the initiation of protein synthesis. Here, we report the cloning and characterization of a novel gene, LceIF1, from a potentially interesting forage grass, Leymus chinensis (Trin.). The expression results show that LceIF1 is expressed in most organisms under normal conditions, but the transcription patterns differ under sodic-saline and sodic-alkaline stresses. Sodic-saline stress induced a persistent decrease, and sodic-alkaline stress induced overexpression of LceIF1. Potassic-saline and alkaline stresses did not cause any changes in expression of eIF1. These results indicate that not only pH but also Na+ concentration affects overtranscription of LceIF1. The eIF1 transgenic lines showed relatively high eIF1 expression, resulting in potentially higher stress resistance. Combined with eIF1 transcription in transgenic lines, LceIF1 as a molecular target of salt toxicity is believed to help enhance salt tolerance.
References
Abd El Samad HM, Shaddad MAK (1996) Comparative effect of sodium carbonate, sodium sulphate, and sodium chloride on the growth and related metabolic activities of pea plants. J Plant Nutr 19:717–728
Apse MP, Aharon GS, Sneddeb WA, Blumwald E (1999) Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285:1256–1258
Bieleski RL, Ferguson IB (1983) Physiology and metabolism of phosphate and its compounds. In: Laüchili A, Pirson A (eds) Encyclopedia of plant physiology, vol 15. Springer, Berlin, pp 522–536
Blumwald E (2000) Sodium transport and salt tolerance in plants. Curr Opin Cell Biol 12:431–434
Brownell PF, Crossland CJ (1972) The requirement for sodium by species having the C4 dicarboxylic photosynthetic pathway. Plant Physiol 49:794
Browning KS (2004) Plant translation initiation factors: it is not easy to be green. Biochem Soc Trans 32:589–591
Cui Y, Dinman JD, Kinzy TG, Peltz SW (1998) The Mof2/Sui1 protein is a general monitor of translational accuracy. Mol Cell Biol 18:1506–1516
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Diédhiou CJ, Popova OV, Dietz KJ, Golldack D (2008) The SUI-homologous translation initiation factor eIF-1 is involved in regulation of ion homeostasis in rice. Plant Biol 10:298–309
Hasegawa PM, Bressan RA, Zhu JK, Bonnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499
Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:1–32
Huang Z, Zhu J, Mu X, Lin J (2004) Pollen dispersion, pollen viability and pistil receptivity in Leymus chinensis. Ann Bot 93:295–301
Jin H, Kim HR, Plaha P, Liu SK, Park JY, Piao YZ, Yang ZH, Jiang GB, Kwak SS, An G, Son M, Jin YH, Sohn JH, Lim YP (2008) Expression profiling of the genes induced by Na2CO3 and NaCl stresses in leaves and roots of Leymus chinensis. Plant Sci 175:784–792
Kang JY, Choi HI, Im MY, Kim SY (2002) Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14:343–357
Kyrpides NC, Woese CR (1998) Universally conserved translation initiation factors. Proc Natl Acad Sci USA 98:224–228
Latha R, Salekdeh GH, Bennett J, Swaminathan MS (2004) Molecular analysis of a stress-induced cDNA encoding the translation initiation factor, eIF1, from the salt-tolerant wild relative of rice, Porteresia coarctata. Funct Plant Biol 31:1035–1042
Laursen BS, Sørensen HP, Mortensen KK, Sperling Petersen HU (2005) Initiation of protein synthesis in bacteria. Microbiol Mol Biol Rev 69:101–123
Marschner H (1995) Mineral nutrition of higher plants. Springer, Berlin
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
Niu XM, Bressan RA, Hasgawa PM, Pardo JM (1995) Ion homeostasis in NaCl stress environments. Plant Physiol 109:735–742
Pestova TV, Borukhov S, Hellen CUT (1998) Eukaryotic ribosomes require initiation factors 1 and 1A to locate initiation codons. Nature 394:854–859
Roll-Mecak A, Shin BS, Dever TE, Burley SK (2001) Engaging the ribosome: universal Ifs of translation. Trends Biochem Sci 26:705–709
Schreier MH, Erni B, Staehelin T (1977) Initiation of mammalian protein synthesis: I. Purification and characterization of seven initiation factors. J Mol Biol 116:727–753
Sun YL, Hong SK (2010) Effects of plant growth regulators and l-glutamic acid on shoot organogenesis in the halophyte Leymus chinensis (Trin.). Plant Cell Tissue Organ Cult 100:317–328
Sun YL, Hong SK (2011) Effects of citric acid as an important component of the responses to saline and alkaline stress in the halophyte Leymus chinensis (Trin.). Plant Growth Regul 64:129–139
Trachsel H, Erni B, Schreier MH, Staehelin T (1977) Initiation of mammalian protein synthesis: II. The assembly of the initiation complex with purified initiation factors. J Mol Biol 116:755–767
Yoon HJ, Donahue TF (1992) The sui1 suppressor locus in Saccharomyces cerevisiae encodes a translation factor that functions during tRNA Met i . Mol Cell Biol 12:248–260
Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71
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This work was supported by the Nutraceutical Bio Brain Korea 21 Project Group.
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Sun, YL., Hong, SK. Sensitivity of Translation Initiation Factor eIF1 as a Molecular Target of Salt Toxicity to Sodic-Alkaline Stress in the Halophytic Grass Leymus chinensis . Biochem Genet 51, 101–118 (2013). https://doi.org/10.1007/s10528-012-9546-9
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DOI: https://doi.org/10.1007/s10528-012-9546-9