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Effects of Selenium and Exendin-4 on Glucagon-Like Peptide-1 Receptor, IRS-1, and Raf-1 in the Liver of Diabetic Rats

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Abstract

Selenium and exendin-4 exert antidiabetic effects by unknown mechanisms. Herein, we investigated their effects on the expression of glucagon-like peptide-1 receptor (GLP-1R), insulin receptor substrate-1 (IRS-1), and Raf-1 in the livers of rats with streptozotocin-induced diabetes. Diabetic rats were injected intraperitoneally with exendin-4 (0.03 μg/kg body weight) twice daily or treated with 5 ppm selenium as sodium selenite in drinking water for 4 weeks. Both selenium and exendin-4 reduced the hyperglycemia in diabetic rats. Induction of diabetes mellitus resulted in decreased level of GLP-1R and increased levels of IRS-1 and Raf-1 in the liver. Treatment of diabetic rats with selenium or exendin-4 resulted in increased level of GLP-1R and decreased levels of IRS-1 and Raf-1 in the liver, compared with the levels in diabetic rats. Therefore, the antidiabetic actions of selenium and exendin-4 involve their effects on GLP-1R, IRS-1, and Raf-1 levels in the liver.

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Acknowledgments

This work was supported by a research fund from the National Council for Scientific Research (CNRS), Lebanon, to M.E.M. (Grant No. 01-11-08).

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Authors and Affiliations

  1. Department of Biological and Environmental Sciences, Faculty of Science, Beirut Arab University, Beirut, Lebanon

    Ghinwa M. Barakat & Mohamed E. Moustafa

  2. Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt

    Mohamed E. Moustafa

  3. Department of Physiology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon

    Anwar B. Bikhazi

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  1. Ghinwa M. Barakat
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  2. Mohamed E. Moustafa
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  3. Anwar B. Bikhazi
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Correspondence to Mohamed E. Moustafa.

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Barakat, G.M., Moustafa, M.E. & Bikhazi, A.B. Effects of Selenium and Exendin-4 on Glucagon-Like Peptide-1 Receptor, IRS-1, and Raf-1 in the Liver of Diabetic Rats. Biochem Genet 50, 922–935 (2012). https://doi.org/10.1007/s10528-012-9532-2

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  • DOI: https://doi.org/10.1007/s10528-012-9532-2

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