Abstract
Genetic heterogeneity influencing enzyme activity may change the capacity to repair DNA damage induced by environmental and endogenous factors. This study aims to assess the impact of Lys751Gln (A/C) polymorphism in the XPD gene, encoding an enzyme involved in the nucleotide excision repair pathway, on individual DNA damage. The DNA damage in human lymphocytes (% DNA in the tail) was quantified by means of single-cell gel electrophoresis. Baseline levels of DNA damage significantly differ between AA homozygotes and carriers of the C allele, and the observed differences were not related to age, gender, or smoking status. It seems that the AA variant is associated with enhanced protection against oxidative DNA damage.
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This work was supported by grant no. N404042/32/0945 from the Ministry of Science and Higher Education.
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Wlodarczyk, M., Nowicka, G. XPD Gene rs13181 Polymorphism and DNA Damage in Human Lymphocytes. Biochem Genet 50, 860–870 (2012). https://doi.org/10.1007/s10528-012-9526-0
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DOI: https://doi.org/10.1007/s10528-012-9526-0