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Na+/H+ Exchanger 1 Gene Expression in Tissues of Yellow Chicken

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Abstract

The Na+/H+ exchanger 1 (NHE1) transmembrane protein regulates intracellular pH, cell survival, cell growth, cell differentiation and plays a critical role in the progression of some diseases, including the pathogenesis of J avian leukosis. The chicken is an ideal model to study the function of NHE1 because it has developed highly efficient Na+-absorptive mechanisms in its small and large intestines. To date, there has been no detailed expression analysis to determine NHE1 expression in various tissues of the chicken. We determined the mRNA and protein expression levels of avian NHE1 by real-time quantitative PCR and immunohistochemical analysis. NHE1 mRNA was detected in all chicken tissues examined. Protein expression levels varied widely among tissues and did not always correlate with mRNA expression. Determining the mRNA and protein of NHE1 expression patterns in chicken should help to delineate the NHE1 role in different tissues and its contribution to physiological and pathological processes. These data provide the basis for examining the distinct function of chicken NHE1 compared with its mammalian counterpart.

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Acknowledgments

This study was supported by grants from the program supported by the National Natural Science Foundation of China (Grant No. 30771612), the program supported by the Foundation of NSFC People’s Government of Guangdong Province (Grant No. U0831002), the project supported by the National Chicken Industry Technical System (Grant No. nycytx-42-G3-03) and the Program for Science and Technical Foundation of Guangdong Province (Grant No. 2009A0201006).

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Authors and Affiliations

  1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China

    Zhang-yong Ning, Yu-fu An, Wen-bao Qi, Heng Wang, Jia-qiang Pan, Xin-tao Wu & Ming Liao

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  1. Zhang-yong Ning
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  2. Yu-fu An
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  3. Wen-bao Qi
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  4. Heng Wang
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  5. Jia-qiang Pan
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  6. Xin-tao Wu
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  7. Ming Liao
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Corresponding author

Correspondence to Ming Liao.

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Zhang-yong Ning and Yu-fu An contributed equally to this study.

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Ning, Zy., An, Yf., Qi, Wb. et al. Na+/H+ Exchanger 1 Gene Expression in Tissues of Yellow Chicken. Biochem Genet 50, 227–234 (2012). https://doi.org/10.1007/s10528-011-9464-2

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  • DOI: https://doi.org/10.1007/s10528-011-9464-2

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