Abstract
Expressed sequence tags (ESTs) obtained from two-directional suppression subtractive hybridization (SSH) of cDNA libraries derived from the pleon of the Chinese mitten crab were analyzed using mRNA subtractive hybridization. After cDNA cloning and sequencing, gene expression profiles were examined, focusing on brachyurization. We assembled 211 non-redundant ESTs from SSH library 1 (yielding 130 unique transcripts: 25 consensi, 105 singletons) and 669 from SSH library 2 (195: 51 consensi, 144 singletons). Functional analysis shows that these genes are putatively involved in various cellular processes (33%), ribosomal RNA/proteins (30%), molting (12%), signal transduction (6%), immune factor and stress proteins (6%), development (4%), energy metabolism (3%), and mitochondrial membrane protein, hormone metabolizing, and chaperone (1% each) of other organisms. Some 3% are of unknown function in SSH library 1. The results facilitate the functional study of candidate genes involved in early developmental processes, especially the regulation of brachyurization in this crab.
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Acknowledgments
The authors are indebted to Dongxi Shen for providing the material. We especially thank the reviewers for their critical reading, insightful comments, and suggestions on this manuscript. This work was supported by grants from the National Natural Science Foundation of China (No. 31000954), Priority Academic Program Development of Jiangsu Higher Education Institutions, Nanjing Normal University Innovative Team Project (No. 0319PM0902), Research Funds Provided to New Recruiments of Nanjing Normal University (No. 184070H2B11), and Research Fund for the Doctoral Program of Higher Education of China (New Teachers, Grant No. 20103207120007).
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Li, P., Zha, J., Sun, H. et al. Identification of Differentially Expressed Genes During the Brachyurization of the Chinese Mitten Crab Eriocheir japonica sinensis . Biochem Genet 49, 645–655 (2011). https://doi.org/10.1007/s10528-011-9439-3
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DOI: https://doi.org/10.1007/s10528-011-9439-3