Abstract
Scrapie is a prion disease in sheep and goats. Ribosomal protein SA (RPSA), also called 37 kDa laminin receptor precursor/67 kDa laminin receptor has been demonstrated to be a putative cell surface receptor for prion. To investigate the caprine RPSA, we cloned the full-length coding sequence of the gene of goat and submitted it to GenBank. The length of the open reading frame is 888 bp, encoding 295 amino acids. The putative amino acid sequence is highly similar to that of other mammals. The caprine amino acid sequence of RPSA is shown to be identical to the sequence of species susceptible to scrapie at positions 241, 272, and 291. The phylogenetic tree analysis revealed that the genetic distance between sheep and goat is the smallest. Moreover, RT-PCR results of 11 tissues indicated that RPSA mRNA is expressed in all selected caprine tissues.
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References
Bortoluzzi S, d’Alessi F, Romualdi C, Danieli GA (2001) Differential expression of genes coding for ribosomal proteins in different human tissues. Bioinformatics 17(12):1152–1157
Clausse N, Jackers P, Jares P, Joris B, Sobel ME, Castronovo V (1996) Identification of the active gene coding for the metastasis-associated 37LRP/p40 multifunctional protein. DNA Cell Biol 15(12):1009–1023
Gauczynski S, Peyrin JM, Haik S, Leucht C, Hundt C, Rieger R, Krasemann S, Deslys JP, Dormont D, Lasmezas CI, Weiss S (2001) The 37-kDa/67-kDa laminin receptor acts as the cell-surface receptor for the cellular prion protein. EMBO J 20:5863–5875
Grosso LE, Park PW, Mecham RP (1991) Characterization of a putative clone for the 67-kilodalton elastin/laminin receptor suggests that it encodes a cytoplasmic protein rather than a cell surface receptor. Biochemistry 30(13):3346–3350
Jackers P, Clausse N, Fernandez M-T, Berti A, Princen F, Wewer U, Sobel ME, Castronovo V (1996) Seventeen copies of the human 37 kDa laminin receptor precursor/p40 ribosome-associated protein gene are processed pseudogenes arisen from retropositional events. Biochim Biophys Acta 1305:98–104
Knorr C, Beuermann C, Beck J, Bertram B (2007) Characterization of the porcine multicopy ribosomal protein SA/37-kDa laminin receptor gene family. Gene 395:135–143
Leucht C, Simoneau S, Rey C, Vana K, Rieger R, Lasmezas CI, Weiss S (2003) The 37 kDa/67 kDa laminin receptor is required for PrP(Sc) propagation in scrapie-infected neuronal cells. EMBO Rep 4:290–295
Marcos-Carcavilla A, Calvo JH, Gonzalez C, Serrano C, Moazami-Goudarzi K, Laurent P, Bertaud M, Hayes H, Beattie AE, Lyahyai J, Martin-Burriel I, Torres JM, Serrano M (2008) Structural and functional analysis of the ovine laminin receptor gene (RPSA): possible involvement of the LRP/LR protein in scrapie response. Mamm Genome 19:92–105
Moreno CR, Cosseddu GM, Schibler L, Roig A, Moazami-Goudarzi K, Andreoletti F, Eychenne O, Lajous D, Schelcher F, Cribiu EP, Laurent P, Vaiman D, Elsen JM (2008) Identification of new quantitative trait loci (other than the PRNP gene) modulating the scrapie incubation period in sheep. Genetics 179(1):723–726
Nikles D, Vana K, Gauczynski S, Knetsch H, Ludewigs H, Weiss S (2008) Subcellular localization of prion proteins and the 37-kDa/67-kDa laminin receptor fused to fluorescent proteins. Biochim Biophys Acta 1782:335–340
Papasavva-Stylianou P, Windl O, Saunders G, Mavrikiou P, Toumazos P, Kakoyiannis C (2010) PrP gene polymorphisms in Cyprus goats and their association with resistance or susceptibility to natural scrapie. Vet J. doi:101016/j.tvjl.2009.10.015
Priola SA (1999) Prion protein and species barriers in the transmissible spongiform encephalopathies. Biomed Pharmacother 53(1):27–33
Qiao JW, Su XO, Wang YQ, Yang JM, Kouadir M, Zhou XM, Yang LF, Yin XM, Zhao DM (2008) Cloning and characterization of full-length coding sequence (CDS) of the ovine 37/67-kDa laminin receptor (RPSA). Mol Biol Rep 36:2131–2137. doi:10.1007/s11033-008-9426-x
Qiao JW, Su XO, Li YX, Yang JM, Wang YQ, Kouadir M, Zhou XM, Yang LF, Yin XM, Zhao DM (2009) Variable levels of 37-kDa/67-kDa laminin receptor (RPSA) mRNA in ovine tissues: Potential contribution to the regulatory processes of PrPSc propagation? Anim Biotechnol 20:151–155
Rao CN, Castronovo V, Schmitt MC, Wewer UM, Claysmith AP, Liotta LA, Sobel ME (1989) Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. Biochemistry 28(18):7476–7486
Rieger R, Edenhofer F, Lasmezas CI, Weiss S (1997) The human 37-kDa laminin receptor precursor interacts with the prion protein in eukaryotic cells. Nat Med 3:1383–1388
Van den Broeke A, Van Poucke M, Marcos-Carcavilla A, Hugot K, Hayes H, Bertaud M, Van Zeveren A, Peelman LJ (2010) Characterization of the ovine ribosomal protein SA gene and its pseudogenes. BMC Genomics 11:179–191
Yow HK, Wong JM, Chen HS, Lee CG, Davis S, Steele GD Jr, Chen LB (1988) Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. Proc Natl Acad Sci USA 85(17):6394–6398
Zuber C, Knackmuss S, Rey C, Reusch U, Rottgen P, Frohlich T, Arnold GJ, Pace C, Mitteregger G, Kretzschmar HA, Little M, Weiss S (2008) Single chain Fv antibodies directed against the 37-kDa/67-kDa laminin receptor as therapeutic tools in prion diseases. Mol Immunol 45(1):144–151
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This work was supported by the National Key Scientific and Technological Project (2006BAD0410-06).
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Zhou, R., Li, X., Zheng, H. et al. Molecular Characterization of the Full-Length Coding Sequence of the Caprine Laminin Receptor Gene (RPSA). Biochem Genet 48, 962–969 (2010). https://doi.org/10.1007/s10528-010-9378-4
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DOI: https://doi.org/10.1007/s10528-010-9378-4