Abstract
Genetic variation and structure of six natural populations of Lepidium draba L. from Eastern Anatolia were assessed using random amplified polymorphic DNA (RAPD) markers. For RAPD analysis, 12 primers generated 218 reproducible bands across the six populations analyzed, of which 73 bands (33.3%) were polymorphic. The mean Nei’s gene diversity value for all six populations was 0.1771. Shannon’s information index varied with population (0.2278–0.3082), averaging 0.2608. Analysis of molecular variance (AMOVA) showed that genetic diversity was greater within populations (58.66%) than among populations (30.68%). In addition, the variation between groups was 10.33%. The genetic differentiation among populations (G ST) was 0.3210, indicating that most genetic diversity occurs within populations. Gene flow (Nm) was low, at only 0.5288.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barrett SCH (1992) Genetics of weed invasions. In: Jain SK, Botsford LW (eds) Applied population biology. Kluwer, Dordrecht, pp 91–119
Bon MC, Hurard C, Gaskin J, Risterucci AM (2005) Polymorphic microsatellite markers in polyploid Lepidium draba L. subsp. draba (Brassicaceae) and cross-species amplification in closely related taxa. Mol Ecol Notes 5:68–70
Fedorenko OM, Gritskikh MV (2008) Diversity of Arabidobsis thaliana (L.) Heynh. natural populations at the northern limits of the species range: RAPD analysis. Russ J Genet 44(4):425–428
Gaskin JF, Zhang DY, Bon MC (2005) Invasion of Lepidium draba (Brassicaceae) in the western United States: distributions and origins of chloroplast DNA haplotypes. Mol Ecol 14:2331–2341
Guo HB, Lu BR, Wu QH, Chen JK, Zhou TS (2007) Abundant genetic diversity in cultivated Codonopsis pilosula populations revealed by RAPD polymorphisms. Genet Resour Crop Evol 54:917–924
Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Plant population genetics, breeding and genetic resources. Sinauer Associates, Sunderland, pp 43–46
Li W, Wang B, Wang J (2006) Lack of genetic variation of an invasive clonal plant Eichornia crassipes in China revealed by RAPD and ISSR markers. Aquat Bot 84:176–180
Liang Y, Liu J, Zhang SP, Wang SJ, Guo WH, Wang RQ (2008) Genetic diversity of the invasive plant Coreopsis grandiflora at different altitudes in Laoshan Mountain, China. Can J Plant Sci 88(4):831–837
Lyons KE (1998) Element stewardship abstract for Cardaria draba (L.) Desv., heart-podded hoary cress, Cardaria chalepensis (L.) Hand-Maz., lens-podded hoary cress, and Cardaria pubescens (C. A. Meyer) Jarmolenko, globe-podded hoary cress. The Nature Conservancy, Arlington. http://tncweeds.ucdavis.edu/esadocs/documnts/card_sp.html
Mulligan GA, Frankton C (1962) Taxonomy of the genus Cardaria with particular reference to the species introduced into North America. Can J Bot 40:1411–1425
Nianxi Z, Yubao G, Jinlong W, Anzhi R, Hua X (2006) RAPD diversity of Stipa grandis populations and its relationship with some ecological factors. Acta Ecol Sin 26(5):1312–1319
O’Hanlon PC, Peakal R, Briest DT (2000) A review of new PCR-based genetic markers and their utility to weed ecology. Weed Res 40:239–254
Qasem JR (1993) Allelopathic effects of some common weed species in cereal crops. Dirasat 20B:7–28
Qasem JR (1994) Allelopathic effect of white top (Lepidium draba) on wheat and barley. Allelopath J 1:29–40
Qasem JR (2001) Allelopathic potential of white top and Syrian sage on vegetable crops. Agron J 93:64–71
Qasem JR (2004) Allelopathic plants. 11. Cardaria draba (L.) Desv. Allelopath J 13:165–172
Qian ZQ, Xu L, Wang Y-L, Yang J, Zhao GF (2008) Ecological genetics of Reaumuria soongorica (Pall.) Maxim. population in the oasis-desert ecotone in Fukang, Xinjiang, and its implications for molecular evolution. Biochem Sys Ecol 36:593–601
Reisch C, Anke A, Röhl M (2005) Molecular variation within and between ten populations of Primula farinosa (Primulaceae) along an altitudinal gradient in the northern Alps. Basic Appl Ecol 6:35–45
Rios JLV, Garcia JFE (1998) Catalogo de malezas de Mexico. Universidad Nacional Autónoma de México y el Fondo de Cultura Económica, Mexico DF
Schaal BA, Hayworth DA, Olsen KM, Rauscher JT, Smith WA (1998) Phylogeographic studies in plants: problems and prospects. Mol Ecol 7:465–474
Slatkin M, Barton NH (1989) A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43:1349–1368
Sun K, Chen W, Ma R, Chen X, Li A, Ge S (2006) Genetic variation in Hippophae rhamnoides ssp. sinensis (Elaeagnaceae) revealed by RAPD markers. Biochem Genet 44(5–6):186–197
Sunar S, Aksakal O, Yildirim N, Agar G, Gulluce M, Sahin F (2009) Genetic diversity and relationships detected by FAME and RAPD analysis among Thymus species growing in eastern Anatolia region of Turkey. Rom Biotech Lett 14(2):4313–4318
USDA (2004) PLANTS database. National Plant Data Center, Baton Rouge. http://plants.usda.gov
Wright S (1951) The genetical structure of populations. Ann Eugen 15:323–354
Xia T, Chen SL, Chen SY, Ge XJ (2005) Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers. Biochem Genet 43(3–4):87–101
Yeh FC, Yang R-C, Boyle T (1999) Popgene version 1.31. Microsoft windows-based freeware for population genetic analysis. University of Alberta, Canada
Acknowledgments
This study was supported by grants from the Research Funds appropriated to Atatürk University.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Aksakal, O., Sunar, S., Kaya, Y. et al. Genetic Diversity Within and Among Lepidium draba Populations from Eastern Anatolia Based on RAPD Analysis. Biochem Genet 48, 603–611 (2010). https://doi.org/10.1007/s10528-010-9342-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-010-9342-3