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Genetic Variation of the Peroxisome Proliferator-Activated Receptor α Gene (PPARA) in Chickens Bred for Different Purposes

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Abstract

Peroxisome proliferator-activated receptor α (PPARA) is involved in fatty acid oxidation by upregulating the expression of acyl-coenzyme A oxidase and carnitine palmitoyltransferase. In this study, PPARA gene variations in four chicken breeds (Guyuan, Wenchang, Tibetan, and Hisex) were detected by PCR-SSCP and DNA sequencing. The results indicated six genotypes (AA-EF). When compared with the PPARA reference sequence (GenBank accession no. AF163809), the nucleotide sequences of genotypes AA, BB, AB, and CC revealed silent mutations in the three Chinese breeds. The nucleotide sequences of genotypes DD and EF in Hisex showed several frame-shift mutations, implying variations involving five alleles of the PPARA gene in chicken breeds. In addition, the distribution of genotype frequency within the PPARA gene was significantly different in the four breeds studied, implying that this locus would probably be an effective marker in marker-assisted selection for layer, meat-and-egg, and broiler breeds.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (no. 30460097) and the Youth Foundation of Northwest A&F University. We thank X. L. Liu and C. Huang at the Hainan chicken-breeding farm (P.R. China) for their excellent care of the Wenchang chicken. We also acknowledge the workers at the Guyuan, Wenchang, and Tibetan chicken-breeding farms in Ningxia, Hainan, and Tibet provinces of China for helping to collect blood samples.

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Authors and Affiliations

  1. College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, China

    J. Q. Zhang, H. Chen, X. L. Liu & Y. L. Gu

  2. Applied Research and Development Center for New Technology, Ningxia University, Yinchuan, Ningxia, 750021, China

    Z. J. Sun

  3. Department of Animal Husbandry and Veterinary Medicine, College of Tibet Agriculture and Animal Husbandry, Tibet University, Linzhi, Tibet, 860000, China

    Y. Z. Qiang-Ba

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  1. J. Q. Zhang
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  2. H. Chen
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  3. Z. J. Sun
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  4. X. L. Liu
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  5. Y. Z. Qiang-Ba
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  6. Y. L. Gu
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Correspondence to H. Chen.

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Zhang, J.Q., Chen, H., Sun, Z.J. et al. Genetic Variation of the Peroxisome Proliferator-Activated Receptor α Gene (PPARA) in Chickens Bred for Different Purposes. Biochem Genet 48, 465–471 (2010). https://doi.org/10.1007/s10528-009-9329-0

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  • DOI: https://doi.org/10.1007/s10528-009-9329-0

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