Abstract
Genetic variation of insulin-like growth factor binding protein-3 was analyzed in 17 pig breeds (14 native Chinese and 3 European). Using PCR-single strand conformation polymorphism, we found a polymorphism in intron 2, and this SNP was the combined mutation of G897T-G903A-C911T. The Chinese breeds carried a higher TAT/TAT genotype frequency (over 50%), except for Bamei (22%), Yujiang Black (0.0%), and Erhualian (10.0%); the European breeds had a higher GGC/GGC genotype frequency (Large White 1.67%, Landrace 13.89%, Duroc 0.0%). The allelic frequency of TAT in Chinese breeds was over 50%, except for Yujiang Black (12.5%); the allelic frequency of GGC was over 50% in all European breeds. The effect of genotype on 43 performance traits was investigated in one population (Lantang × Landrace). Pigs with the TAT/TAT genotype had higher B-point and C-point back-fat thickness than pigs with the GGC/GGC genotype. The TAT/TAT pigs also scored higher in meat color than the GGC/GGC pigs. These results implied that IGFBP-3 may affect meat quality and carcass traits.
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Acknowledgments
This work was supported by the State Key Basic Research and Development Program (G200000161) and the Doctoral Initiation Fund of Jiangxi Agricultural University (2004). The authors thank Prof. Chen Yaosheng, Huanan Agricultural University, Guangdong, China, for his kind donation of the Jinhua × Pietrain population family.
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Wang, W., Meng, Q., Hu, X. et al. Genetic Variation and Association of Insulin-Like Growth Factor Binding Protein-3 with Performance in Swine. Biochem Genet 47, 315–321 (2009). https://doi.org/10.1007/s10528-009-9230-x
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DOI: https://doi.org/10.1007/s10528-009-9230-x