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A Comparative Analysis of Molecular Diversity of Erect Milkvetch (Astragalus adsurgens) Germplasm from North China Using RAPD and ISSR Markers

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References

  • Archak S, Gaikwad AB, Gautam D, Rao EVVB, Swamy KRM, Karihaloo JL (2003) Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR and AFLP) for genetic analysis of cashew (Anacardium occidentale L.) accessions of India. Genome 46:362–369

    Article  PubMed  CAS  Google Scholar 

  • Boronnikoval SV, Kokaeva ZG, Gostimsky SA, Dribnokhodova OP, Tikhomirova NN (2007) Analysis of DNA polymorphism in a relict Uralian species, large-flowered foxglove (Digitalis grandiflora Mill.), using RAPD and ISSR markers. Rus J Genet 43:530–535

    Article  Google Scholar 

  • Carriero F, Fontanazza G, Cellinil F, Giorio G (2002) Identification of simple sequence repeats (SSRs) in olive (Olea europaea L.). Theor Appl Genet 104:301–307

    Article  PubMed  CAS  Google Scholar 

  • Chen MJ, Jia SX (2002) Chinese feeding plant. Beijing, Chinese Agricultural Press, pp 401–402

    Google Scholar 

  • Chen ZH, Huang LK, Zhang XQ, Wang ZG (2006) Establishment of Astragalus adsurgens′ ISSR-PCR optimal conditions using orthogonal optimization method. J Anhui Agric Sci 34:2980–2982

    Google Scholar 

  • Doyle J (1991) DNA protocols for plants—CTAB total DNA isolation. In: Hewitt GM, Johnston A (eds) Molecular techniques in taxonomy. Springer, Berlin, pp 283–293

    Google Scholar 

  • Fernández M, Figueiras A, Benito C (2002) The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor Appl Genet 104:845–851

    Article  PubMed  Google Scholar 

  • Flora of China Editorial Committee (1994) Flora of China, vol 42. Science Press, Beijing, pp 272–273

    Google Scholar 

  • Hao B, Li W, Mu LC, Li Y, Zhang R, Tang MX, Bao WK (2006) A study of conservation genetics in Cupressus chengiana, an endangered endemic of China, Using ISSR Markers. Biochem Genet 44:29–43

    Article  Google Scholar 

  • Kochieva EZ, Ryzhova NN, Legkobit MP, Khadeeva NV (2006) RAPD and ISSR analyses of species and populations of the genus Stachys. Russ J Genet 42:723–727

    Article  CAS  Google Scholar 

  • Kuznetsova OI, Ash OA, Hartina GA, Gostimskij SA (2005) RAPD and ISSR analyses of regenerated pea Pisum sativum L. plants. Russ J Genet 41:60–65

    CAS  Google Scholar 

  • Li Q, He T, Xu Z (2005) Genetic evaluation of the efficacy of en Situ and Ex Situ conservation of Parashorea chinensis (Dipterocarpaceae) in Southwestern China. Biochem Genet 43:387–406

    Article  PubMed  CAS  Google Scholar 

  • Li RF, Li C, Su JK (2001) Genetic diversity of Astragalus adsurgens Pall. germplasm by RAPD analysis. Acta Agrestia Sinica 9:171–175

    Google Scholar 

  • Loarce Y, Gallego R, Ferrer E (1993) A comparative analysis of genetic relationships between rye cultivars using RFLP and RAPD markers. Euphytic 88:107–115

    Article  Google Scholar 

  • Mamuris Z, Sfougaris AI, Stamatis C, Suchentrunk F (2002) Assessment of genetic structure of Greek brown hare (Lepus europaeus) populations based on variation in random amplified polymorphic DNA (RAPD). Biochem Genet 40:323–338

    Article  PubMed  CAS  Google Scholar 

  • Mehrnia M, Zarre S, Sokhan-Sanj A (2005) Intra- and inter-specific relationships within the Astragalus microcephalus complex (Fabaceae) using RAPD. Biochem Syst Ecol 33:149–158

    Article  CAS  Google Scholar 

  • Milbourne I, Rbonda M, Bradsbaw JE, Baird E, Bonar N, Provan J, Powell W, Waugh R (1997) Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Mol Breed 3:127–136

    Article  CAS  Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individual. Genetics 89:583–590

    PubMed  Google Scholar 

  • Powell W, Morgante M (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:225–238

    Article  CAS  Google Scholar 

  • Qian W, Ge S, Hong DY (2001) Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theor Appl Genet 102:440–449

    Article  CAS  Google Scholar 

  • Rohlf FJ (2001) NTSYS-pc numerical taxonomy and multivariate analysis system. Exeter Software, New York, USA

    Google Scholar 

  • Souframanien J, Gopalakrishna T (2004) A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theor Appl Genet 109:1687–1693

    Article  PubMed  CAS  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalskil JA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  PubMed  CAS  Google Scholar 

  • Zhang DF, Chen SL, Chen SY, Zhang DJ, Gao Q (2007) Patterns of genetic variation in Swertia przewalskii, an endangered endemic species of the Qinghai–Tibet plateau. Biochem Genet 45:33–50

    Article  PubMed  CAS  Google Scholar 

  • Zhao KG, Zhou MQ, Chen LQ (2007) Genetic diversity and discrimination of Chimonanthus praecox (L.) Link germplasm using ISSR and RAPD markers. HortScience 42:1046–1311

    Google Scholar 

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)—anchored polymerase chain reaction amplification. Genetics 20:176–183

    CAS  Google Scholar 

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Acknowledgments

This study was supported by the Species Resource Preservation Special Disbursement Financial Fund (No. 070401), the National Basic Research Program (973 Program) in China (No. 2007CB108907), and the Program for New Century Excellent Talents in University of China (No. NCET-04-0909).

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Authors and Affiliations

  1. Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Yaan, 625014, People’s Republic of China

    Lin-kai Huang & Xin-quan Zhang

  2. National Center of Preservation and Utilization of Genetic Resources of Animal and Forage, National Animal Husbandry and Service, Beijing, 100094, People’s Republic of China

    Zhi-hong Chen, Zhi-gang Wang & Chou-sheng Liu

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  1. Lin-kai Huang
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  2. Zhi-hong Chen
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  3. Xin-quan Zhang
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  4. Zhi-gang Wang
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  5. Chou-sheng Liu
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Correspondence to Zhi-hong Chen or Xin-quan Zhang.

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Huang, Lk., Chen, Zh., Zhang, Xq. et al. A Comparative Analysis of Molecular Diversity of Erect Milkvetch (Astragalus adsurgens) Germplasm from North China Using RAPD and ISSR Markers. Biochem Genet 47, 92–99 (2009). https://doi.org/10.1007/s10528-008-9209-z

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