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High ISSR Variation in 14 Surviving Individuals of Euryodendron excelsum (Ternstroemiaceae) Endemic to China

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Abstract

Euryodendron excelsum is a critically endangered Ternstroemiaceae species endemic to southern China, with only 14 individuals surviving in Ba Jia Zhen of Yangchun, Guangdong Province. Intersimple sequence repeat (ISSR) markers were used to assess genetic variation and relationships among these individuals. Population genetic parameters were estimated by a Bayesian approach as well as conventional methods. Of the 225 loci generated by 21 primers, 147 (65.33%) were polymorphic. Compared with other species of Theaceae and related families, a high level of genetic variation was identified in E. excelsum (Nei’s gene diversity, 0.2458; Shannon’s index, 0.3626). An unweighted pair group method with arithmetic mean (UPGMA) dendrogram showed that the 14 individuals were mainly clustered into three groups, a conclusion further supported by principal coordinate analysis. Based on these results, a management and conservation strategy for E. excelsum was proposed.

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Acknowledgments

The authors thank Dr. Bobby A. Brown of Plant Science Group, Division of Biochemistry and Molecular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, for advice and revision of English. We also thank Yueshi Ye from South China Botanical Garden, Chinese Academy of Sciences, for assistance with the collection of plant materials. This work was supported by grants from the National Natural Science Foundation of China (grant no. 30271094), the 100 Talent Project of the Chinese Academy of Sciences (grant no. 0729281F02), and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry of China (grant no. [2003] 406).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, P.R. China

    Yingjuan Su & Yufei Sun

  2. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, P.R. China

    Ting Wang

  3. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, P.R. China

    Huagu Ye

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  1. Yingjuan Su
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  2. Ting Wang
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Correspondence to Ting Wang.

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Su, Y., Wang, T., Sun, Y. et al. High ISSR Variation in 14 Surviving Individuals of Euryodendron excelsum (Ternstroemiaceae) Endemic to China. Biochem Genet 47, 56–65 (2009). https://doi.org/10.1007/s10528-008-9206-2

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  • DOI: https://doi.org/10.1007/s10528-008-9206-2

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