Abstract
PCR-SSCP and DNA sequencing methods were applied to reveal three novel single nucleotide polymorphisms (SNPs) in exon 2 of the POU1F1 gene in 963 Chinese cattle belonging to eight breeds. Among them, a silent SNP (NM_174579:c.545G > A) detected by TaqI endonuclease is described. Frequencies of the POU1F1-G allele varied from 0.685 to 1.000. The association of TaqI polymorphism with growth traits was analyzed in 251 Nanyang cattle. No significant associations of the TaqI polymorphism with body weight and average daily gain for different growth periods (6, 12, 18, and 24 months old) were observed (P > 0.05), as well as for body sizes (P > 0.05).
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Acknowledgments
This work was funded by the National 863 Program of China (No. 2006AA10Z197), the National Key Technology R&D Program (2006BAD01A10-5), the Chinese National Natural Science Foundation (No. 30771544), the Outstanding Talents Foundation of Northwest A&F University (No. 01140101), and the doctoral program for Northwest A&F University (Xianyong Lan).
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Chuanying Pan and Xianyong Lan contributed equally to this work.
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Pan, C., Lan, X., Chen, H. et al. A TaqI PCR-RFLP Detecting a Novel SNP in Exon 2 of the Bovine POU1F1 Gene. Biochem Genet 46, 424–432 (2008). https://doi.org/10.1007/s10528-008-9160-z
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DOI: https://doi.org/10.1007/s10528-008-9160-z