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Novel SNPs of the mtDNA ND5 Gene and Their Associations with Several Growth Traits in the Nanyang Cattle Breed

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Abstract

Polymorphism of the mtDNA ND5 gene was detected by PCR-SSCP and DNA sequencing methods in 714 individuals from six Chinese cattle breeds. The results showed that at the P2 locus, two kinds of haplotypes, named A and B, with three SNPs (T12900C, A12923T, C12924T) were detected. The frequencies of haplotype A in six breeds were 0.041–0.167. Polymorphism of the ND5 gene was shown to be associated with growth in the Nanyang breed. Individuals with haplotype B had greater hucklebone width than those with haplotype A when 6 months old (P < 0.01), as well as better body height, body length, body weight, and average daily gain at 6 months (P < 0.05). Therefore, haplotype B of the ND5 gene was likely to have a positive effect on growth traits at 6 months in the Nanyang breed.

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Acknowledgments

This study was supported by the National 863 Program of China (No. 2006AA10Z197), National Natural Science Foundation of China (No. 30471238), National Key Technology R&D Program (No. 2006BAD01A10-5), Innovative Foundation of Outstanding Talent from Henan Province (No. 0521001900), Sustaining Program for Topnotch Persons of Northwest A&F University (No. 01140101), and Natural Science Foundation of Xuzhou Normal University.

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Authors and Affiliations

  1. College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, P.R. China

    Bao Zhang, Hong Chen, Liushuai Hua, Chunlei Zhang, Xiangtao Kang, Chuanying Pan, Xianyong Lan & Chuzhao Lei

  2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu, 221116, P.R. China

    Hong Chen

  3. Research Center of Cattle Engineering Technology in Henan, Zhengzhou, Henan, 450003, P.R. China

    Xinzhuang Wang

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  1. Bao Zhang
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  2. Hong Chen
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  3. Liushuai Hua
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  4. Chunlei Zhang
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  5. Xiangtao Kang
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  6. Xinzhuang Wang
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  7. Chuanying Pan
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  8. Xianyong Lan
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  9. Chuzhao Lei
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Correspondence to Hong Chen.

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Zhang, B., Chen, H., Hua, L. et al. Novel SNPs of the mtDNA ND5 Gene and Their Associations with Several Growth Traits in the Nanyang Cattle Breed. Biochem Genet 46, 362–368 (2008). https://doi.org/10.1007/s10528-008-9152-z

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