Abstract
The 26S ATP-dependent protease is composed of a 20S catalytic proteasome and two PA700 regulatory modules; it plays a central role in many regulatory pathways, such as cell cycle regulation, differentiation, and apoptosis. The PA700 complex is composed of multiple subunits, including at least six related ATPases and approximately 15 non-ATPase polypeptides. PSMC1 (proteasome 26S subunit, ATPase, 1) is one of these ATPases. In this study, we amplified a fragment of 507 bp from intron 9 of the bovine PSMC1 gene and found a SNP (G/A) at position 216 in the PCR fragment. Genotyping of 138 animals from four beef breeds revealed that the average frequency for allele A (G-base) was 0.4271 (0.3269–0.5517); for allele B (A-base) it was 0.5729 (0.4483–0.6731). This SNP is significantly associated with average daily feed intake (P < 0.01), average daily gain, finishing average daily gain, body length, ratio of feed to meat, backfat thickness, and loin-muscle area (P < 0.05). Our experimental data showed that animals with an AA genotype have a significantly lower food intake, grow faster, are longer in the body, and have less backfat and bigger loin muscle; hence, their ratio of feed to meat is significantly lower. We believe that the PSMC1 SNP is a potential candidate marker for marker-assisted selection in these traits. We also found that the bovine PSMC1 gene was expressed mainly in lung, testis, and spleen. In addition, we mapped the bovine PSMC1 gene on BTA10 by an RH mapping method.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Everts-van der Wind A, Kata SR, Band MR, Rebeiz M, Larkin DM, Everts RE, Green CA, Liu L, Natarajan S, Goldammer T, Lee JH, McKay S, Womack JE, Lewin HA (2004) A 1463 gene cattle-human comparative map with anchor points defined by human genome sequence coordinates. Genome Res 14(7):1424–1437
Guo H, Liu WS, Takasuga A, Eyer K, Landrito E, Xu SZ, Gao X, Ren HY (2005) Mapping of the CCK, PSMC2, PSMC4, PSMD1, CPB1 and PSPH genes in cattle. Anim Genet 37:72–89
Itoh T, Watanabe T, Ihara N, Mariani P, Beattie CW, Sugimoto Y, Takasuga A (2005) A comprehensive radiation hybrid map of the bovine genome comprising 5593 loci. Genomics 85(4):413–424
Lin L, Liu B, Yu M, Yerle M, Fan B, Yang J, Li K (2004) Radiation hybrid mapping of the pig ALDOA, ALDOB and ALDOC genes to SSC3, SSC1 and SSC12. Anim Genet 35(1):66–67
Pan PW, Zhao SH, Yu M, Liu B, Xiong TA, Li K (2003) Identification of differentially expressed genes in the longissimus dorsi muscle tissue between Duroc and Erhualian pigs by mRNA differential display. Asian-Aust J Anim Sci 16(7):1066–1070
Slonim D, Kruglyak L, Stein L, Lander E (1997) Building human genome maps with radiation hybrids. J Comput Biol 4(4):487–504
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J. (2002). Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index. Mamm Genome 13(7):373–379
Tanahashi N, Suzuki M, Fujiwara T, Takahashi E, Shimbara N, Chung CH, Tanaka K (1998) Chromosomal localization and immunological analysis of a family of human 26S proteasomal ATPases. Biochem Biophys Res Commun 243(1):229–232
Wang YF, Yu M, Yerle M, Liu B, Zhao SH, Xiong TA, Fan B, Li K (2003) Mapping of genes encoding four ATPase and three non-ATPase components of the pig 26S proteasome. Anim Genet 34(5):393–395
Wu X, Yu M, Liu B, Yerle M, Zhao SH, Wang YF, Fan B, Li K (2004) Mapping of three porcine 20S proteasome genes using the ImpRH panel. Cytogenet. Genome Res 106:142E
Acknowledgment
The project was supported by the National High Technology Research and Development Plan (2002AA242011) of China (863 Program).
Author information
Authors and Affiliations
Corresponding author
Additional information
The sequences of the 140 bp fragment (accession no. DQ111992) and the 507 bp fragment (accession no. DQ139315) have been submitted to GenBank
Rights and permissions
About this article
Cite this article
Guo, H., Liu, WS., Takasuga, A. et al. Mapping, Expression, and Association Study of the Bovine PSMC1 Gene. Biochem Genet 46, 347–355 (2008). https://doi.org/10.1007/s10528-008-9151-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-008-9151-0