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Genetic Heterogeneity of the Blue Swimming Crab (Portunus pelagicus) in Thailand Determined by AFLP Analysis

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Abstract

Genetic diversity and population differentiation of the blue swimming crab (Portunus pelagicus) in Thailand, originating from Ranong and Krabi located in the Andaman Sea (west) and Chanthaburi, Prachuap Khiri Khan, and Suratthani located in the Gulf of Thailand (east), were examined by AFLP analysis. High genetic diversity of P. pelagicus in Thai waters was found (N = 72). The four primer combinations generated 227 AFLP fragments, and the percentage of polymorphic bands in each geographic sample was 66.19–94.38%. The mean genetic distance between pairs of samples was 0.1151–0.2440. Geographic heterogeneity analyses using the exact test and F ST-based statistics between all pairwise comparisons were statistically significant (P < 0.01), indicating a fine-scale level of intraspecific population differentiation of Thai P. pelagicus. The estimated number of migrants per generation (N e m) was 0.26–0.76, suggesting restricted gene flow levels of P. pelagicus in Thai waters.

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Acknowledgments

This research is supported by the National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand, awarded to SK and BK.

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Authors and Affiliations

  1. Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Klong 1, Klong Luang, Pathum Thani, 12120, Thailand

    Sirawut Klinbunga & Bavornlak Khamnamtong

  2. Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Sirawut Klinbunga, Bavornlak Khamnamtong & Piamsak Menasveta

  3. Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Kannika Khetpu

  4. Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Piamsak Menasveta

Authors
  1. Sirawut Klinbunga
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  2. Kannika Khetpu
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  3. Bavornlak Khamnamtong
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  4. Piamsak Menasveta
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Correspondence to Sirawut Klinbunga.

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Klinbunga, S., Khetpu, K., Khamnamtong, B. et al. Genetic Heterogeneity of the Blue Swimming Crab (Portunus pelagicus) in Thailand Determined by AFLP Analysis. Biochem Genet 45, 725–736 (2007). https://doi.org/10.1007/s10528-007-9110-1

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  • DOI: https://doi.org/10.1007/s10528-007-9110-1

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