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Molecular cloning, mapping, and tissue expression of the porcine cluster of differentiation 14 (CD14) gene

Biochemical Genetics volume 45, pages 459–468 (2007)Cite this article

Abstract

CD14 plays an important role in initiating the innate response to lipopolysaccharide from Gram-negative bacteria. The gene and corresponding cDNA of porcine CD14 were sequenced and characterized. The porcine CD14 gene consists of two exons and a short intron (80 bp) located immediately after the ATG translation start codon. This structure is very similar to the CD14 gene of human, rat, mouse, rabbit, horse, and cow. The sequence of the porcine CD14 protein is 59–76% identical to that of rat, mouse, rabbit, human, horse, and cow CD14 protein. A highly conserved structure of the CD14 protein with respect to the leucine-rich repeats domain and the N-glycosylation sites was observed between species. Porcine CD14 was assigned to porcine chromosome 2q21 by a radiation hybrid panel. Using RT-PCR analysis, porcine CD14 transcripts were detected in liver, spleen, thymus, white matter, and skeletal muscle.

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Acknowledgments

This study was supported by the Key project of the National Natural Science Foundation of China (Grant No. 30430500) and the National Key Basic Research Program (Grant No. 2006CB102104). We also gratefully acknowledge M. Yerle (INRA, Toulouse) for the pig radiation hybrid (IMpRH) panels.

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  1. Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, P.R. China

    Xiao-Tian Qiu, Yu-Hua Li, HeJun Li, Ying Yu & Qin Zhang

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  1. Xiao-Tian Qiu
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  2. Yu-Hua Li
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  3. HeJun Li
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  4. Ying Yu
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  5. Qin Zhang
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Correspondence to Qin Zhang.

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Qiu, XT., Li, YH., Li, H. et al. Molecular cloning, mapping, and tissue expression of the porcine cluster of differentiation 14 (CD14) gene. Biochem Genet 45, 459–468 (2007). https://doi.org/10.1007/s10528-007-9088-8

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  • DOI: https://doi.org/10.1007/s10528-007-9088-8

Keywords

  • CD14
  • Innate immunity
  • Leucine-rich repeats
  • Radiation hybrid panel
  • Gene expression