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Genetic Polymorphism of β-Lactoglobulin Gene in Native Turkish Sheep Breeds

The genetic polymorphism of the β-lactoglobulin gene was investigated in three native Turkish sheep breeds. The study was carried out on 108 sheep (29 Kıvırcık, 38 Gökçeada, and 41 Sakız) by means of PCR-RFLP methods. Two genetic variants (A and B) and three genotypes (AA, AB, and BB) of β-lactoglobulin have been identified. The gene frequencies of β-LG A and B were 0.7759 and 0.2241 in Kıvırcık, 0.7632 and 0.2368 in Gökçeada, and 0.9756 and 0.0244 in Sakız breeds, respectively. The populations were in Hardy–Weinberg equilibrium in all samples from the three breeds.

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  1. Aliev, G. A., and Koloteva, R. S. (1975). Geneticheskoe raznoobrazie beta-laktoglobulina V moloke ovets. Anim. Breed. Abstr. 43:400.

    Google Scholar 

  2. Amigo, L., Recio, I., and Ramos, M. (2000). Genetic polymorphism of ovine milk proteins: Its influence on technological properties of milk. Int. Dairy J. 10:135.

    Article  CAS  Google Scholar 

  3. Anton, I., Zsolnai, A., and Fesüs, L. (1999). Identification of the variant C of β-lactoglobulin in sheep using a polymerase chain reaction—Restriction fragment length polymorphism method. J. Anim. Breed. Genet. 116:525.

    Article  CAS  Google Scholar 

  4. Aschaffenburg, R., and Drewry, J. (1955). Occurrence of different β-lactoglobulins in cow’s milk. Nature 176:218.

    PubMed  Article  CAS  Google Scholar 

  5. Baranyi, M., Bözse, Z. S., Buchberger, J., and Krause, I. (1993). Genetic polymorphism of milk proteins in Hungarian spotted and Hungarian grey cattle: A possible new genetic variant of β-lactoglobulin. J. Dairy Sci. 76:630.

    CAS  Article  Google Scholar 

  6. Barillet, F., Arranz, J. J., and Carta, A. (2005). Mapping quantitative trait loci for milk production and genetic polymorphism of milk proteins in dairy sheep. Genet. Sel. Evol. 37(Suppl. 1):109.

    Article  CAS  Google Scholar 

  7. Cubric-Curic, V., Feligini, M., Lukac-Havranek, J., Curic, I., and Enne, G. (2002). Genetic polymorphism of lactoglobulin in native sheep from the Island of Pag. Food Tech. Biotechnol. 40(1):75.

    Google Scholar 

  8. Elyasi, G., Shodja, J., Nassiry, M. R., Tahmasebi, A., Pirahary, O., and Javanmard, A. (2004). Polymorphism of β-lactoglobulin gene in Iranian sheep breeds using PCR-RFLP. In Proceedings of the Joint Agriculture and Natural Resources Symposium, Tabriz-Ganja, May 14–16, 2004.

  9. Erhardt, G. (1988). Genetic polymorphisms of α-lactalbumin and β-lactoglobulin in sheep milk. Anim. Genet. 20(Suppl. 1):76.

    Google Scholar 

  10. Erhardt, G. (1989). Evidence for a third allele at the β-lactoglobulin (β-Lg) locus of sheep and its occurrence in different breeds. Anim. Genet. 20:197.

    PubMed  CAS  Article  Google Scholar 

  11. Erhardt, G., Godovac-Zimmermann, J., and Conti, A. (1989). Isolation and complete primary sequence of a new ovine wild-type beta-lactoglobulin C. Biol. Chem. Hoppe Seyler 370:757.

    PubMed  CAS  Google Scholar 

  12. Feligini, M., Parma, P., Aleandri, R., Greppi, G. F., and Enne, G. (1998). PCR-RFLP test for direct determination of β-lactoglobulin genotype in sheep. Anim. Genet. 29:473.

    PubMed  CAS  Google Scholar 

  13. Kolde, H. J., and Braunitzer, G. (1983). The primary structure of ovine beta-lactoglobulin, 2: Discussion and genetic aspects. Milchwissenschaft 38:70.

    CAS  Google Scholar 

  14. Marziali, A. S., and Ng-Kwai-Hang, K. F. (1986). Effects of milk composition and genetic polymorphism on cheese composition. J. Dairy Sci. 69:2533.

    CAS  Google Scholar 

  15. Mercier, J. C., and Vilotte, J. L. (1993). Structure and function of milk protein genes. J. Dairy Sci. 76:3079.

    PubMed  CAS  Article  Google Scholar 

  16. Ng-Kwai-Hang, K. F., Monardes, H. G., and Hayes, J. F. (1990). Association between genetic polymorphisms of milk proteins and production traits during three lactations. J. Dairy Sci. 73:3414.

    CAS  Article  Google Scholar 

  17. Pagnacco, G., and Caroli, A. (1987). Effect of casein and β-lactoglobulin genotypes on renneting properties of milks. J. Dairy Res. 54:479.

    CAS  Article  Google Scholar 

  18. Recio, I., Fernandez-Fournier, A., Martin-Alvarez, P. J., and Ramos, M. (1997). β-Lactoglobulin polymorphism in ovine breeds: Influence on cheese-making properties and milk composition. Lait 77:259.

    CAS  Google Scholar 

  19. Yeh, F., Yang, R. C., and Boyle, T. (2000). Popgene (v. 1.32), Microsoft Windows-based freeware for Population Genetic Analysis. Retrieved from

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This work was supported by grants from the Scientific Research Projects Council of Uludag University (project no. 2002-74), Bursa, Turkey.

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Correspondence to Cengiz Elmaci.

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Elmaci, C., Oner, Y. & Balcioglu, M.S. Genetic Polymorphism of β-Lactoglobulin Gene in Native Turkish Sheep Breeds. Biochem Genet 44, 376–381 (2006).

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  • Turkish sheep breeds
  • genetic polymorphism
  • β-lactoglobulin gene