This is a preview of subscription content, log in via an institution to check access.
REFERENCES
Anson, D. S., Taylor, J. A., Bielicki, J., Harper, G. S., Peters, C., Gibson, G. J., and Hopwood, J. J. (1992). Correction of human mucopolysaccharidosis type-VI fibroblasts with recombinant N-acetylgalactosamine-4-sulphatase. Biochem. J. 284:789–794.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 7:248–254.
Brown, C. A., and Mahuran, D. J. (1991). Active arginine residues in beta-hexosaminidase. Identification through studies of the B1 variant of Tay-Sachs disease. J. Biol. Chem. 25:15855–15862.
Chung, S., Andersson, T., Sonntag, K. C., Bjorklund, L., Isacson, O., and Kim, K. S. (2002). Analysis of different promoter systems for efficient transgene expression in mouse embryonic stem cell lines. Stem Cells 20:139–145.
Furth, P. A., St Onge, L., Boger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H., and Hennighausen, L. (1994). Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc. Natl. Acad. Sci. U.S.A. 91:9302–9306.
Goldman, L. A., Cutrone, E. C., Kotenko, S. V., Krause, C. D., and Langer, J. A. (1996). Modifications of vectors pEF-BOS, pcDNA1, and pcDNA3 result in improved convenience and expression. Biotechniques 21:1013–1015.
Gopalkrishnan, R. V., Christiansen, K. A., Goldstein, N. I., DePinho, R. A., and Fisher, P. B. (1999). Use of the human EF-1alpha promoter for expression can significantly increase success in establishing stable cell lines with consistent expression: A study using the tetracycline-inducible system in human cancer cells. Nucleic Acids Res. 27:4775–4782.
Gravel, R., Kaback, M. M., Proia, R. L., Sandhoff, K., Suzuki, K., and Suzuki, K. (2001). The GM2 gangliosidoses. In Scriver, C. R., Beaudet, A. L., Sly, W. S., Valle, D. (eds.), The Metabolic and Molecular Bases of Inherited Disease, 8th edn., McGraw-Hill, New York, pp. 3827–3876.
Kim, D. W., Uetsuki, T., Kaziro, Y., Yamaguchi, N., and Sugano, S. (1990). Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene 91:217–223.
Kim, S. Y., Lee, J. H., Shin, H. S., Kang, H. J., and Kim, Y. S. (2002). The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter. J. Biotechnol. 93:183–187.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 15:680–685.
Mahuran, D. J. (1999). Biochemical consequences of mutations causing the GM2 gangliosidosis. Biochem. Biophys. Acta 1455:105–138.
Özkara, H. A., and Sandhoff, K. (2003). Characterization of two Turkish beta-hexosaminidase mutations causing Tay-Sachs disease. Brain Dev. 25(3):191–194.
Sinici, I., Tropak, M. B., Mahuran, D. J., and Özkara, H. A. (2004). Assessing the severity of the small inframe deletion mutation in the α-subunit of β-hexosaminidase A found in the Turkish population by reproducing it in the more stable β-subunit. J. Inherit. Metab. Dis. 27:747–756.
Teschendorf, C., Warrington, K. H. Jr., Siemann, D. W., and Muzyczka, N. (2002). Comparison of the EF-1 alpha and the CMV promoter for engineering stable tumor cell lines using recombinant adeno-associated virus. Anticancer Res. 22:3325–3330.
Tokushige, K., Moradpour, D., Wakita, T., Geissler, M., Hayashi, N., and Wands, J. R. (1997). Comparison between cytomegalovirus promoter and elongation factor-1 alpha promoter-driven constructs in the establishment of cell lines expressing hepatitis C virus core protein. J. Virol. Methods 64:73–80.
Zhang, S., Bagshaw, R., Hilson, W., Oho, Y., Hinek, A., Clarke, J. T., and Callahan, J. W. (2000). Characterization of beta-galactosidase mutations Asp332 → Asn and Arg148 → Ser, and a polymorphism, Ser532 → Gly, in a case of GMI gangliosidosis. Biochem. J. 348:621–632.
ACKNOWLEDGMENTS
We thank Amy Leung for excellent technical assistance. This work was supported by the Scientific and Technical Research Council of Turkey (TUBITAK).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sinici, I., Zarghooni, M., Tropak, M.B. et al. Comparison of HCMV IE and EF-1 Promoters for the Stable Expression of β-Subunit of Hexosaminidase in CHO Cell Lines. Biochem Genet 44, 168–175 (2006). https://doi.org/10.1007/s10528-006-9016-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-006-9016-3