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Ribosomal DNA ITS-1 Intergenic Spacer Polymorphism in Triatomines (Triatominae, Heteroptera)

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Abstract

The length polymorphism of ribosomal DNA ITS-1 intergenic spacer was analyzed in eight species of triatomines belonging to Triatoma, Rhodnius, and Panstrongylus genera. The analyzed species were Rhodnius domesticus, R. neivai, R. robustus, Triatoma brasiliensis, T. infestans, T. vitticeps, Panstrongylus megistus, and P. herreri. These insects are vectors of Chagas' disease, one of the most prominent public health problems among South American countries. This work allowed the differentiation between species of the Triatomini and Rhodniini tribes through the analysis of ITS-1 length polymorphism by PCR and RFLP techniques. The species of the Triatoma and Panstrongylus genera presented an amplified ITS-1 fragment between 600 and 1000 bp, whereas Rhodnius presented a less variable ITS-1 length fragment, around 300 bp, which could reflect the monophyletic origin of the Rhodniini tribe. Species belonging to this genus were further differentiated by RFLP with HaeIII and AluI endonucleases. Our results corroborate the hypothesis of polyphyletic origin in this group of insects and contribute to knowledge about evolutionary relationships in triatomines.

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References

  • Altclas, J., Jaimovich, G., Milovic, V., Klein, F., and Feldman, L. (1996). Chagas' disease after bone marrow transplantation. Bone Marrow Transpl. 23:447–448.

    Google Scholar 

  • Baffi, M. A., and Ceron, C. R. (2002). Molecular analysis of the rDNA ITS-1 intergenic spacer in Drosophila mulleri, D. arizonae, and their hybrids. Biochem. Genet. 40:411–421.

    Article  PubMed  Google Scholar 

  • Bargues, M. D., Marcilla, A., Ramsey, J. M., Dujardin, J. P., Schofield, C. J., and Mas-Coma, S. (2000). Nuclear rDNA-based molecular clock of the evolution of Triatominae (Hemiptera: Reduviidae), vectors of Chagas' disease. Mem. Inst. Oswaldo Cruz 95:567–573.

    Article  PubMed  Google Scholar 

  • Beebe, N. W., Ellis, R. D., Cooper, R. D., and Saul, A. (1999). DNA sequence analysis of the ribosomal DNA ITS2 region for the Anopheles pinctulatus group of mosquitoes. Insect Mol. Biol. 8:381–390.

    Article  PubMed  Google Scholar 

  • Blangero-Williams, S., Vandeberg, J. L., Blangero, J., and Teixeira, A. R. L. (1997). Genetic epidemiology of seropositivity for Trypanosoma cruzi infection in rural Goias, Brazil. Am. J. Trop. Med. Hyg. 57(5):538–543.

    PubMed  Google Scholar 

  • Carcavallo, R. U., Jurberg, J., and Lent, H. (1999). Phylogeny of the triatominae. In Carcavallo, R. U., Girón, I. G., Jurberg, J., and Lent. Rio de Janeiro, L. (eds.), Atlas of Chagas' Disease Vectors in the Americas, Vol. 3, pp. 925–965.

  • Cocciolone, S. M., and Cone, K. C. (1993). Pl-Bb, an anthocyanin regulatory gene of maize that leads to variegated pigmentation. Genetics 135:575–588.

    PubMed  Google Scholar 

  • Colli, W., and Alves, M. J. M. (1998). Chagas um repensar de abordagens experimentais. Médi HC-FMUSP 1(4):74–77.

    Google Scholar 

  • Dias, J. C. P., Silveira, A. C., and Schofield, C. J. (2002). The impact of Chagas control in Latin America: A review. Mem. Inst. Oswaldo Cruz 97:603–612.

    Article  PubMed  Google Scholar 

  • Gaulel, S. A., and Kirchhoff, L. V. (1996). Risk factors for Trypanosoma cruzi infection in California blood donors. Transfusion 36:227–231.

    Article  PubMed  Google Scholar 

  • Gaunt, M., and Miles, M. (2000). The ecotopes and evolution of triatomine bugs (Triatominae) and their associated trypanosomas. Mem. Inst. Oswaldo Cruz 95(4):557–565.

    Article  PubMed  Google Scholar 

  • Gorla, D. E., Dujardin, J. P., and Schofield, C. J. (1997). Biosystematics of old world triatominae. Acta Trop. 63:127–140.

    Article  PubMed  Google Scholar 

  • Hypsa, V., Tietz, D. F., and Zrzavy, J. (2002). Phylogeny and biogeography of Triatominae (Hemiptera: Reduviidae). Molecular evidence of a new world origin. Mol. Phylogen. Evol. 23:447–457.

    Article  Google Scholar 

  • Jobst, J., King, K., and Hemleben, V. (1998). Molecular evolution of the internal transcribed spacer (ITS1 and ITS2) and phylogenetic relationships among species of the family Cucurbitaceae. Mol. Phylogen. Evol. 9(2):204–219.

    Article  Google Scholar 

  • Lyman, D. F., Monteiro, F. A., Escalante, A. A., Cordon-Rosales, C., Wesson, D. W., Dujardin, J.-P., and Beard, C. B. (1999). Mitochondrial DNA sequence variation among triatomine vectors of Chagas' disease. Am. J. Trop. Med. Hyg. 60(3):377–386.

    PubMed  Google Scholar 

  • Marcilla, A., Bargues, M. D., Abad-Franch, F., Panzera, F., Carcavallo, R. U., Galvão, C., Jurberg, J., Miles, M. A., Dujardin, J. P., and Mas-Coma, S. (2002). Nuclear rDNA ITS-2 sequences reveal polyphyly of Panstrongylus species (Hemiptera: Reduviidae: Triatominae), vectors of Trypanosoma cruzi. Infect. Genet. Evol. 1:225–235.

    Article  PubMed  Google Scholar 

  • Marcilla, A., Bargues, M. D., Ramsey, J. M., Magallon-Gastelum, E., Schettino, P. M. S., Abad-Franch, F., Dujardin, J. P., Schofield, C. J., and Mas-Coma, S. (2001). The ITS-2 of the nuclear rDNA as a molecular marker for populations, species, and phylogenetic relationships in Triatomianae (Hemiptera: Reduviidae), vectors of Chagas' disease. Mol. Phylogen. Evol. 18:136–142.

    Article  Google Scholar 

  • Monti, J. R., Chilton, N. B., Bao-Zhen, Q., and Gasser, R. B. (1998). Specific amplification of Necator americanus or Ancylostoma duodenale DNA by PCR using markers in ITS-1 rDNA and its implications. Mol. Cell. Probes 12:71–78.

    Article  PubMed  Google Scholar 

  • Onyabe, D. Y., and Conn, J. E. (1999). Intragenomic heterogeneity of a ribosomal DNA spacer (ITS2) varies regionally in the neotropical malaria vector Anopheles nuneztovari (Dipetera : Culicidae). Insect Mol. Biol. 8:435–442.

    Article  PubMed  Google Scholar 

  • Polanco, C., González, A. I., Fuente, A., and Dover, G. A. (1998). Multigene family of ribosomal DNA in Drosophila melanogaster reveals contrasting patterns of homogenization for IGS and ITS spacer regions: A possible mechanism to resolve this paradox. Genetics 149:243–256.

    PubMed  Google Scholar 

  • Proft, J., Maier, W. A., and Kampen, H. (1999). Identification of six sibling species of the Anopheles maculipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay. Parasitol. Res. 85:837–843.

    Article  PubMed  Google Scholar 

  • Reiche, E. M. V., Inouye, M. M. Z., Pontello, R., Morimoto, H. K., Itow Jankevicius, S., Matsuo, T., and Jankevicius, J. V. (1996). Seropositivity for anti-Trypanosoma cruzi antibodies among blood donors of the Hospital Universitário Regional do Norte do Paraná, Londrina, Brazil (1). Rev. Inst. Med. Trop. S. Paulo 38(3):233–240.

    PubMed  Google Scholar 

  • Schofield, C. J. (1988). Biosystematics of the Triatominae. Biosyst. Haematophag. Insects 37:284–312.

    Google Scholar 

  • Schofield, C. J. (2000). Trypanosoma cruzi: The vector-parasite paradox. Mem. Inst. Oswaldo Cruz 95(4): 535–544.

    Article  PubMed  Google Scholar 

  • Schofield, C. J., and Dujardin, J. P. (1999). Theories on the evolution of Rhodnius. Actual Biol. (Medellin) 21:183–197.

    Google Scholar 

  • Stothard, J. R., Yamamoto, Y., Cherchi, A., Garcia, A. L., Valente, S. A. S., Schofield, C. J., and Miles, M. A. (1998). A preliminary survey of mitochondrial sequence variation in Triatominae (Hemiptera: Reduviidae) using polymerase chain reaction-based single strand conformational polymorphism (SSCP) analysis and direct sequencing. Bull. Entomol. Res. 88:553–560.

    Google Scholar 

  • Toma, T., Miyagi, I., Crabtree, M. B., and Miller, B. R. (2000). Identification of Culex vishnui subgroup (Diptera : Culicidae) mosquitoes from the Ryukyu Archipelago, Japan: Development of a species-diagnostic polymerase chain reaction assay based on sequence variation in ribosomal DNA spacer. J. Med. Entomol. 37:554–558.

    PubMed  Google Scholar 

  • Vidigal, T. H. D. A., Spatz, L., Nunes, D. N., and Simpson, A. J. G. (1998). Biomphalaria spp: Identification of the intermediate snail hosts of Schistosoma mansoni by polymerase chain reaction amplification and restriction enzyme digestion of the ribosomal RNA gene intergenic spacer. Exp. Parasitol. 89:180–187.

    Article  PubMed  Google Scholar 

  • Wu, C. C. N., and Fallon, A. M. (1998). Analysis of a ribosomal DNA intergenic spacer region from the yellow fever mosquito Aedes aegypti. Insect Mol. Biol. 7(1):19–29.

    Article  PubMed  Google Scholar 

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  1. Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual de Paulista, Rua Cristovão Colombo 2265, 15054.000, São José do Rio Preto, SP, Brazil

    Ester Tartarotti & Carlos Roberto Ceron

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  1. Ester Tartarotti
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  2. Carlos Roberto Ceron
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Correspondence to Ester Tartarotti.

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Tartarotti, E., Ceron, C.R. Ribosomal DNA ITS-1 Intergenic Spacer Polymorphism in Triatomines (Triatominae, Heteroptera). Biochem Genet 43, 365–373 (2005). https://doi.org/10.1007/s10528-005-6776-0

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  • DOI: https://doi.org/10.1007/s10528-005-6776-0

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