A device for treating in vitro cultures of stem cells and neuroblasts with weak electric fields has been developed. The purpose of the treatment is to control the process of cell differentiation, as well as the growth and orientation of neurites. The structure of the device and the electric circuit of its output stage are described, together with the main technical specifications and the operation of the device.
Similar content being viewed by others
References
Song, B., Gu, Y., Pu, J., Reid, B., Zhao, Z., and Zhao, M., “Application of direct current electric fields to cells and tissues in vitro and modulation of wound electric field in vivo,” Nat. Protoc., 2, 1479–489 (2007).
Li, L., El-Hayek, Y. H., Liu, B., Chen, Y., et al., “Direct-current electrical field guides neuronal stem/progenitor cell migration,” Stem Cells, 6, 2193–2200 (2008).
Yao, L., McCaig, C. D., and Zhao, M., “Electrical signals polarize neuronal organelles, direct neuron migration, and orient cell division,” Hippocampus, 19, 855–868 (2009).
Thompson, B. C., Richardson, R. T., Moulton, S. E., et al., “Conducting polymers, dual neurotrophins and pulsed electrical stimulation – Dramatic effects on neurite outgrowth,” J. Contr. Rel., 141, 161–167 (2010).
Pires, F., Ferreira, Q., Rodrigues, C. A., Morgado, J., and Ferreira, F. C., “Neural stem cell differentiation by electrical stimulation using a cross-linked PEDOT substrate: Expanding the use of biocompatible conjugated conductive polymers for neural tissue engineering,” Biochim. Biophys. Acta., 1850, No. 6, 1158–1168 (2015).
Graves, M. S., Hassell, T., Beier, B. L., Albors, G. O., and Irazoqui, P. P., “Electrically mediated neuronal guidance with applied alternating current electric fields,” Ann. Biomed. Eng., 39, 1759–1767 (2011).
Tang-Schomer, M. D., “3D axon growth by exogenous electrical stimulus and soluble factors,” Brain Res., 1678, No. 1, 288–296 (2018).
Xiong, G. M., Do, A. T., Wang, J. K., Yeoh, C. L., Yeo, K. S., and Choong, C., “Development of a miniaturized stimulation device for electrical stimulation of cells,” J. Biol. Eng., 9, No. 14, 1–10 (2015).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Meditsinskaya Tekhnika, Vol. 54, No. 5, Sep.-Oct., 2020, pp. 1-3.
Rights and permissions
About this article
Cite this article
Domanskiy, V.L., Koshelev, S.M. & Sobakin, I.A. A Generator of Weak Electric Fields for Treatment of In Vitro Cultures of Stem and Neuronal Cells. Biomed Eng 54, 299–302 (2021). https://doi.org/10.1007/s10527-021-10026-w
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10527-021-10026-w