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Automated Reticulocyte Counting in Peripheral Blood Smears

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Biomedical Engineering Aims and scope

The results of reticulocyte counting obtained using the Vision Hema® Reticulocytes (Vision Hema® RET) software are presented and compared to the results obtained by manual reticulocyte counting. Blood samples with pronounced reticulocytosis (main group, n = 15) and samples with normal cell count (control group, n = 15) are analyzed. Use of a scanner analyzer allows the reticulocyte counting time in the samples of the main group to be reduced by 90.5% (from 202.3 ± 44.7 to 106.3 ± 18.3 s); in the control group it is reduced by 56% (from 155.1 ± 38.0 to 99.9 ± 16.8 s). The results of reticulocyte counting using the Vision Hema® RET software do not differ from the light microscopy data in the main group (p = 0.211) and in the control group (p = 0.53). The Spearman’s correlation coefficient R is 0.977. The results of evaluation of the within-run precision (CVWR) demonstrate high reproducibility of reticulocyte count as compared to the manual method in cases of both normal (9.45% against 20.5%) and high (4.43% against 8.63%) reticulocyte counts. The Vision Hema® RET soft-ware is recommended for use in clinical diagnostic laboratories.

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Authors and Affiliations

  1. Academician E. A. Wagner Perm State Medical University, Ministry of Health of the Russian Federation, Perm, Russia

    D. Yu. Sosnin, O. G. Kubarev & N. V. Pozdin

  2. OOO West Medica, Perm, Russia

    L. S. Onjanova & B. F. Falkov

Authors
  1. D. Yu. Sosnin
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  2. L. S. Onjanova
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  3. B. F. Falkov
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  4. O. G. Kubarev
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  5. N. V. Pozdin
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Corresponding author

Correspondence to D. Yu. Sosnin.

Additional information

Translated from Meditsinskaya Tekhnika, Vol. 51, No. 4, Jul.-Aug., 2017, pp. 15-18.

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Sosnin, D.Y., Onjanova, L.S., Falkov, B.F. et al. Automated Reticulocyte Counting in Peripheral Blood Smears. Biomed Eng 51, 249–253 (2017). https://doi.org/10.1007/s10527-017-9724-5

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  • DOI: https://doi.org/10.1007/s10527-017-9724-5

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