Abstract
In many tropical countries, studies to determine the shelf-life of biopesticides for commercialization and storage without refrigeration have been conducted under mild (≤ 25 °C) temperature regimes. Are these temperatures representative of non-refrigerated rural deposits? To answer this question, we set up trials in five Brazilian geographical regions. Representative temperatures were estimated using two distinct approaches: (1) calculation of the mean kinetic temperature (MKT) inside packages stored in rural deposits for up to 19 months, and (2) comparison of germination counts of fungi (Beauveria bassiana and Trichoderma spp.) kept under these conditions with those of conidia kept at constant temperatures. MKTs calculated for deposits located in the Northern, Northeastern, Central-Western, Southeastern, and Southern regions were 28.0, 26.7, 23.6, 22.2, and 21.7 °C, respectively. For all regions, MKTs calculated for the long-term trials were very close (≤ 0.5 °C) to the values calculated for the year 2013. However, in certain periods, MKTs in the South and Southeast deposits were near 25 °C, whereas variations in other regions were minimal (≤ 0.6 °C). The less precise estimate of representative temperatures in rural deposits, based on germination counts of B. bassiana and T. harzianum, were in the 23.8–35.4 and 24.0–27.5 °C ranges, respectively, depending on the geographical region. Our results indicate that guidelines that propose 30 °C to be adopted for stability tests for pharmaceutical and food products in tropical countries must also be applied to biopesticides intended for country-wide commercialization and storage under non-refrigerated conditions in Brazil.
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References
AGROFIT (2021) Sistema de agrotóxicos fitossanitários, do Ministério da Agricultura, Pecuária e Abastecimento. http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons. Accessed 01 July 2021
Alvares CA, Stape JL, Sentelhas PC, Gonçalves JLM, Sparovek G (2013) Köppen’s climate classification map for Brazil. Meteorol Z 22:711–728
ANVISA (2005) Resolução—RE nº 1 de 29 de julho de 2005. Guia para a realização de estudos de estabilidade. https://bvsms.saude.gov.br/bvs/saudelegis/anvisa/2005/res0001_29_07_2005.html. Accessed 12 July 2021
ANVISA (2018) Guia para determinação de prazos de validade de alimentos. Guia nº 16/2018. https://docero.com.br/doc/8vxcc0. Accessed 12 July 2021.
ANVISA (2019) Resolução—RDC nº 318. https://www.in.gov.br/web/dou/-/resolucao-rdc-n-318-de-6-de-novembro-de-2019-226513805. Accessed 12 July 2021
Bott RF, Oliveira WP (2007) Storage conditions for stability testing of pharmaceuticals in hot and humid regions. Drug Dev Ind Pharm 33:393–401
Chandler D, Davidson G, Pell JK, Ball BV, Shaw K, Sunderland KD (2000) Fungal biocontrol of Acari. Biocontrol Sci Technol 10:357–384
Faria M, Hotchkiss JH, Wraight SP (2012) Application of modified atmosphere packaging (gas flushing and active packaging) for extending the shelf life of Beauveria bassiana conidia at high temperatures. Biol Control 61:78–88
Faria M, Lopes RB, Souza DA, Wraight SP (2015) Conidial vigor vs. viability as predictors of virulence of entomopathogenic fungi. J Invertebr Pathol 125:68–72
Faria M, Martins I, Souza DA, Mascarin GM, Lopes RB (2017) Susceptibility of the biocontrol fungi Metarhizium anisopliae and Trichoderma asperellum (Ascomycota: Hypocreales) to imbibitional damage is driven by conidial vigor. Biol Control 107:87–94
Glare TR, Caradus J, Gelernter W, Jackson T, Keyhani N, Kohl J, Marrone P, Morin L, Stewart A (2012) Have biopesticides come of age? Trends Biotechnol 30:250–258
Glare TR, Jurat-Fuentes JL, O’Callaghan MO (2017) Basic and applied research: entomopathogenic bacteria. In: Lacey LA (ed) Microbial control of insect and mite pests. Elsevier, Amsterdam, pp 47–67
Grimm W (1993) Storage conditions for stability testing in the EC, Japan and USA; the most important market for drug products. Ind Pharm 19:2795–2830
Grimm W (1998) Extension of the international conference on harmonisation tripartite guidelines for stability testing of new drug substances and products to countries of climatic zones III and IV. Drug Dev Ind Pharm 24:313–325
Grzywacz D (2017) Basic and applied research: Baculovirus. In: Lacey LA (ed) Microbial control of insect and mite pests. Elsevier, Amsterdam, pp 27–46
Harman GE (2011) Trichoderma—not just for biocontrol anymore. Phytoparasitica 39:103–108
Haynes JD (1971) Worldwide virtual temperature for product stability testing. J Pharm Sci 60:927–929
Huynh-Ba K, Zahn M (2009) Understanding ICH guidelines applicable to stability testing. In: Huynh-Ba K (ed) Handbook of stability testing in pharmaceutical development: regulations, methodologies, and best practices. Springer, pp 9–41
Hilbe JM (2015) Practical guide for logistic regression. CRC, New York
International Safe Transit Association (2002) ISTA temperature project—data summary. Report. East Lansing, MI, USA, p. 11. https://docplayer.net/5515399-Ista-temperature-project-data-summary.html. Accessed 12 July 2021
Jones KA, Burges HD (1998) Technology of formulation and application. In: Burges HD (ed) Formulation of microbial biopesticides. Beneficial microorganisms, nematodes and seed treatments. Kluwer Academic Publishers, Dordrecht, pp 2–30
Kassa A (2003) Development and testing of mycoinsecticides based on submerged spores and aerial conidia of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) for control of locusts, grasshoppers and storage pests. PhD, Georg-August-Universität Göttingen (Germany)
Lacey LA, Grzywacz D, Shapiro-Ilan DI, Frutos R, Brownbridge M, Goettel MS (2015) Insect pathogens as biological control agents: back to the future. J Invertebr Pathol 132:1–41
Lopes RB, Martin I, Souza DA, Faria M (2013) Influence of some parameters on the germination assessment of mycopesticides. J Invertebr Pathol 112:236–242
Marques EJ, Alves SB (1996) Otimização de formulações na preservação de conídios de Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metschn.) Sorok. em diferentes condições de armazenamento. Arq Biol Tecnol 39:861–877
Menaria BL (2007) Bioherbicides: an eco-friendly approach to weed management. Curr Sci India 92:10–11
R Development Core Team (2012) R: a language and environment for statistical computing. R Foundation for Statistical Computing. http://www.r-project.org.
Sandhu SS, Rajak RC, Agarwal GP (1993) Studies on prolonged storage of Beauveria bassiana conidia: effects of temperature and relative humidity on conidial viability and virulence against chikpea borer, Helicoverpa armigera. Biocontrol Sci Technol 3:47–53
Shoham J (2020) The rise of biological products in the crop protection and plant nutrition markets. Outlooks Pest Manage 31:129–131
Silva RZ da (2006) Formulação e armazenamento de conídios de Beauveria bassiana (Bals.) Vuill. PhD, Universidade Estadual de Londrina (Brazil)
Waterman KC (2009) Understanding and predicting pharmaceutical product shelf-life. In: Huynh-Ba K (ed) Handbook of stability testing in pharmaceutical development: regulations, methodologies, and best practices. Springer, New York, pp 115–135
WHO (2004) Stability studies in a global environment. Geneva meeting. Working document QAS/05.146 with comments, 10 p. https://www.who.int/medicines/services/expertcommittees/pharmprep/QAS05_146Stabilitywithcomments.pdf. Accessed 12 July 2021
Zahn M (2009) Global stability practices. In: Huynh-Ba K (ed) Handbook of stability testing in pharmaceutical development: regulations, methodologies, and best practices. Springer, New York, pp 43–91
Acknowledgements
Sincere thanks to the owners of farms and to the partners that collaborated in the delivery/collection of mycopesticide packages in pesticide deposits in different regions of Brazil, including Eduardo José da Silva (EMATER/RO), Marco Tamai (Universidade do Estado da Bahia); Todi Sujuki (Fazenda Nossa Senhora Aparecida); Antônio Celso Pinheiro, Renato Ledesma Aleixo, and Ariclenes Anibal Ballarotti (Koppert Biological Systems; formerly Itaforte Bioprodutos); Gilmar Robert de Jesus (Fundação ABC); Pedro Luiz Aiçar de Suss (Fazenda Caracol); and Roberto Teixeira Alves and Anselmo de Castro (Embrapa Cerrados).
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MF: Planning; collected the data, wrote the paper. LAMP: Conceived and designed the analysis; performed the analysis. DAS: Collected the data. RBL: Planning, wrote the paper.
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Faria, M., Palhares, L.A.M., Souza, D.A. et al. What would be representative temperatures for shelf-life studies with biopesticides in tropical countries? Estimates through long-term storage of biocontrol fungi and calculation of mean kinetic temperatures. BioControl 67, 213–224 (2022). https://doi.org/10.1007/s10526-021-10126-2
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DOI: https://doi.org/10.1007/s10526-021-10126-2