The symbiotic bacterial communities of phytophagous arthropods are affected by host species and feeding habits, but such effects have been poorly studied in natural enemies. Here, we investigated the entire bacterial microbiome of two species of predatory mites, Neoseiulus californicus and Neoseiulus barkeri, feeding on three types of diets (artificial diet, pollen and their natural prey, the spider mite Tetranychus urticae) by high-throughput sequencing of the 16S rRNA gene. We found that the bacterial diversity of predatory mites feeding on artificial diet was significantly different from pollen and spider mite feeding groups in both N. californicus and N. barkeri, while bacterial diversity also differed strikingly between the two species even when feeding on the same artificial diet. This finding suggests that the bacterial community of predatory mites is determined by both species and diet. Alphaproteobacteria and Gammaproteobacteria were the two dominant bacterial classes in both predatory mite species, except for N. californicus feeding on artificial diet. The bacterium Bosea sp. was detected in all samples as the core microbial species in predatory mites. Additionally, we discuss whether Bradyrhizobiaceae and Rhodobacteraceae bacteria could be used as probiotics in the artificial diet of N. californicus for better mass rearing.
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We appreciate Dr. Tobin Hammer for comments and editing. This research was supported by the National Natural Science Foundation of China (Grant No. 32070402) and Agricultural Science and Technology Innovation Program, CAAS “Protection and Application of Insect Natural Enemies”.
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
There are no ethical concerns regarding the organisms and the topic of this research.
Handling Editor: Eric Riddick.
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Yan, J., Zhang, B., Li, G. et al. Bacterial communities in predatory mites are associated with species and diet types. BioControl (2021). https://doi.org/10.1007/s10526-021-10112-8
- 16S rRNA
- Feeding habit
- Artificial diet