In China, the fall webworm, Hyphantria cunea, causes enormous losses. The potential of entomopathogenic fungi (EPF) to control pest insects has been demonstrated in the laboratory and the field. In this study, we isolated and identified EPF from the surfaces of H. cunea larvae cadavers, and we investigated their efficacy against fall webworm larvae under bioassay conditions in the laboratory. Isaria javanica BE01 conidial suspensions with concentrations between 1 × 104 and 1 × 108 conidia ml−1 were shown to have marked effects on the larval mortality (the 3rd, 4th, and 5th instar larvae) over a 15 days period. However, the effect of the instar on the mortality was not always significant, except during the late stages (10–14 days) at low concentrations (1 × 104–1 × 106 conidia ml−1) or the early stages (1–5 days) at high concentrations (1 × 107–1 × 108 conidia ml−1). Otherwise, the mortality of the 3rd and 4th instar larvae exceeded 85% at 1 × 108 conidia ml−1. The present study suggests that I. javanica BE01 is a potential biological control agent of H. cunea.
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This study was supported by grants from the National Key Research and Development Program of China (2017YFD0600104).
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All the authors declare that they have no conflict of interest.
This article does not contain any studies on humans or animals performed by any of the authors.
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Wang, W., Zhou, L., Dong, G. et al. Isolation and identification of entomopathogenic fungi and an evaluation of their actions against the larvae of the fall webworm, Hyphantria cunea (Drury) (Lepidoptera: Arctiidae). BioControl 65, 101–111 (2020). https://doi.org/10.1007/s10526-019-09982-w
- Isaria javanica
- Fall webworm
- Biological control
- Larval instar
- Conidial suspensions concentration