, Volume 63, Issue 6, pp 833–842 | Cite as

Bacillus sp. strain M10 as a potential biocontrol agent protecting chili pepper and tomato fruits from anthracnose disease caused by Colletotrichum capsici

  • Prapasri Srikhong
  • Kongyuth Lertmongkonthum
  • Rapeewan Sowanpreecha
  • Panan RerngsamranEmail author


Bacillus sp. strain M10 was observed to produce an antifungal protein that inhibits the growth of Colletotrichum capsici, which is the causal agent of anthracnose disease of chili pepper and tomato. Ammonium sulfate precipitation, anion exchange chromatography, and sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the protein was approximately 55.4 kDa. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and a subsequent sequence database search indicated the antifungal protein was most similar to the Bacillus amyloliquefaciens vegetative catalase (KatA) protein. Light microscopy observation revealed that the antifungal protein induced abnormal hyphal elongation and conidial swelling and rupture. The protein considerably inhibited anthracnose development and protected the fruits from C. capsici infection. Thus, Bacillus sp. strain M10 and/or its putative catalase may be useful as a post-harvest biocontrol agent that protects chili pepper and tomato fruits from anthracnose disease caused by C. capsici.


Antifungal Bacillus Catalase Chili Colletotrichum Tomato 



This work was supported by The 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund).


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Copyright information

© International Organization for Biological Control (IOBC) 2018

Authors and Affiliations

  • Prapasri Srikhong
    • 1
  • Kongyuth Lertmongkonthum
    • 1
  • Rapeewan Sowanpreecha
    • 1
  • Panan Rerngsamran
    • 1
    Email author
  1. 1.Department of Microbiology, Faculty of ScienceChulalongkorn UniversityBangkokThailand

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