, Volume 61, Issue 6, pp 639–647 | Cite as

Molecular characterization of parasitoids from armored scales infesting citrus orchards in Corsica, France

  • Margarita C. G. Correa
  • Ferran PaleroEmail author
  • Noémie Dubreuil
  • Laure Etienne
  • Mathieu Hulak
  • Gilles Tison
  • Sylvie Warot
  • Didier Crochard
  • Nicolas Ris
  • Philippe Kreiter


Armored scales (Hemiptera: Diaspididae) are important pests in citrus orchards worldwide. Augmentative releases of Aphelinidae wasps (Hymenoptera) have been performed in Corsica, France to control the California Red Scale (Aonidiella aurantii (Maskell, 1879)) and the arrowhead scale (Unaspis yanonensis (Kuwana, 1923)), but biological control of armored scales requires the identification of their parasitoids to evaluate their potential as biological control agents. In order to circumvent this issue, parasitoids emerging from four armored scale species were characterized through DNA barcoding. All the parasitoids identified belong to the Aphelinidae (Hymenoptera) and included a total of five Aphytis (including A. melinus), four Encarsia (including cryptic species) and one Ablerus (hyperparasitoid) species. Host-specificity was found to be strong among primary parasitoids, with Encarsia inquirenda Silvestri, 1930 and an unidentified Encarsia being the sole taxa able to parasitize the two subfamilies (Aspidiotinae and Diaspidinae).


Diaspididae Armored scale Parasitoid DNA barcoding Cryptic species 



Thanks are due to the AREFLEC and INRA colleagues that helped with sampling, and to the producers and technical advisors in Plant Protection that gave access to their orchards. Special thanks are due to the INRA-BPI team for their patience and support. This work was funded by the ONEMA through the ECOPHYTO program of the Cors’Aphy Project. MC and FP received financial support from the Marie Curie FP7 IAPP Project “Colbics” #324475. FP also acknowledges support by the project CHALLENGEN (CTM2013-48163) of the Spanish Government.

Supplementary material

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Supplementary material 1 (DOCX 26 kb)


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Copyright information

© International Organization for Biological Control (IOBC) 2016

Authors and Affiliations

  • Margarita C. G. Correa
    • 1
  • Ferran Palero
    • 1
    • 4
    Email author
  • Noémie Dubreuil
    • 2
  • Laure Etienne
    • 2
  • Mathieu Hulak
    • 2
  • Gilles Tison
    • 3
  • Sylvie Warot
    • 1
  • Didier Crochard
    • 1
  • Nicolas Ris
    • 1
  • Philippe Kreiter
    • 1
  1. 1.INRA, Univ. Nice Sophia Antipolis, CNRS, UMR 1355-7254 Institut Sophia AgrobiotechSophia AntipolisFrance
  2. 2.AREFLEC, Corsic’AgropôleSan GiulianoFrance
  3. 3.INRA Centre de San Giuliano, Unité citrus, Pôle agronomiqueSan GiulianoFrance
  4. 4.Centre d’Estudis Avançats de Blanes (CEAB-CSIC)BlanesSpain

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