, Volume 61, Issue 3, pp 257–267 | Cite as

Trichoderma atroviride SC1 prevents Phaeomoniella chlamydospora and Phaeoacremonium aleophilum infection of grapevine plants during the grafting process in nurseries

  • Ilaria Pertot
  • Daniele Prodorutti
  • Andrea Colombini
  • Luca Pasini


Phaeoacremonium aleophilum (Pal) and Phaeomoniella chlamydospora (Pch) are commonly and consistently found in the wood discoloration of the three tracheomycotic syndromes of esca and are thus considered the causal agents of this phaeotracheomycotic complex. Infections commonly occur in vineyards or derive from infected mother plants. However the grafting process in nurseries can pose an additional risk of infections. Trichoderma atroviride SC1, applied at the hydration, callusing and pre-planting stages, effectively controlled infection of Pal and Pch, hydration treatments proving the most effective. The viability of conidia of T. atroviride SC1 in the suspension used to soak the grapevine cuttings did not change within the first 72 h at temperatures of between 5 and 15 °C and it was possible to re-use the suspension at least four times within 48 h without losing viability, making the treatment a practical and valuable measure for nurseries.


Wood diseases Vitis vinifera Biofungicide Esca Brown wood streaking Petri disease Grapevine leaf stripe disease 



This study was financially supported by the EU INNOVA project (FP7-People-2012-IAPP, grant agreement 324416). We thank Denise Ress, Oscar Giovannini, Carmela Sicher, Christian Cainelli and Veronica Leoni for their help with lab and field experiments.


  1. Arzanlou M, Narmani A (2014) Multiplex PCR for specific identification and determination of mating type in Togninia minima (anamorph Phaeoacremonium aleophilum), a causal agent of esca disease of grapevine. Phytopathol Mediterr 53:240–249Google Scholar
  2. Bertsch C, Ramírez-Suero M, Magnin-Robert M, Larignon P, Chong J, Abou-Mansour E, Spagnolo A, Clément C, Fontaine F (2013) Grapevine trunk diseases: complex and still poorly understood. Plant Pathol 62:243–265CrossRefGoogle Scholar
  3. Cardoso F, Nascimento T, Oliveira H (2014) Development of a monoclonal antibody TAS-ELISA assay for detection of Phaeomoniella chlamydospora. Phytopathol Mediterr 53:194–201Google Scholar
  4. Di Marco S, Osti F (2007) Applications of Trichoderma to prevent Phaeomoniella chlamydospora infections in organic nurseries. Phytopathol Mediterr 46:73–83Google Scholar
  5. Eskalen A, Gubler WD (2001) Association of spores of Phaeomoniella chlamydospora, Phaeoacremonium inflatipes, and Pm. aleophilum with grapevine cordons in California. Phytopathol Mediterr 40:S429–S432Google Scholar
  6. Fleurat-Lessard P, Luini E, Berjeaud JM, Roblin G (2014) Immunological detection of Phaeoacremonium aleophilum, a fungal pathogen found in esca disease. Eur J Plant Pathol 139:137–150CrossRefGoogle Scholar
  7. Fourie PH, Halleen F (2004) Proactive control of Petri disease of grapevine through treatment of propagation material. Plant Dis 88:1241–1245CrossRefGoogle Scholar
  8. Fourie PH, Halleen F (2006) Chemical and biological protection of grapevine propagation material from trunk disease pathogens. Eur J Plant Pathol 116:255–265CrossRefGoogle Scholar
  9. Fourie PH, Halleen F, Jvd Vyver, Schreuder W (2001) Effect of Trichoderma treatments on the occurrence of decline pathogens in the roots and rootstocks of nursery grapevines. Phytopathol Mediterr 40:S473–S478Google Scholar
  10. Graham AB, Melton LD, Smith BG (2007) Effect of inoculation with Phaeomoniella chlamydospora on mortality, graft strength and polyphenol content of young grapevines. Phytopathol Mediterr 46:119Google Scholar
  11. Gramaje D, Armengol J (2011) Fungal trunk pathogens in the grapevine propagation process: potential inoculum sources, detection, identification, and management strategies. Plant Dis 95:1040–1055CrossRefGoogle Scholar
  12. Gramaje D, Armengol J (2012) Effects of hot-water treatment, post-hot-water-treatment cooling and cold storage on the viability of dormant grafted grapevines under field conditions. Aust J Grape Wine Res 18:158–163CrossRefGoogle Scholar
  13. Gramaje D, Armengol J, Salazar D, Lopez-Cortes I, Garcia-Jimenez J (2009a) Effect of hot-water treatments above 50 °C on grapevine viability and survival of Petri disease pathogens. Crop Prot 28:280–285CrossRefGoogle Scholar
  14. Gramaje D, Aroca A, Raposo R, Garcia-Jimenez J, Armengol J (2009b) Evaluation of fungicides to control Petri disease pathogens in the grapevine propagation process. Crop Prot 28:1091–1097CrossRefGoogle Scholar
  15. Kotze C, van Niekerk J, Mostert L, Halleen F, Fourie P (2011) Evaluation of biocontrol agents for grapevine pruning wound protection against trunk pathogen infection. Phytopathol Mediterr 50:S247–S263Google Scholar
  16. Larignon P, Dubos B (2000) Preliminary studies on the biology of Phaeoacremonium. Phytopathol Mediterr 39:184–189Google Scholar
  17. Longa CMO, Pertot I, Tosi S (2008) Ecophysiological requirements and survival of a Trichoderma atroviride isolate with biocontrol potential. J Basic Microbiol 48:269–277CrossRefPubMedGoogle Scholar
  18. Mostert L, Groenewald JZ, Summerbell RC, Gams W, Crous PW (2006) Taxonomy and pathology of Togninia (Diaporthales) and its Phaeoacremonium anamorphs. Stud Mycol 54:1–113CrossRefGoogle Scholar
  19. Pierron RJG, Pages M, Couderc C, Compant S, Jacques A, Violleau F (2015) In vitro and in planta fungicide properties of ozonated water against the esca-associated fungus Phaeoacremonium aleophilum. Sci Hortic 189:184–191CrossRefGoogle Scholar
  20. Pouzoulet J, Mailhac N, Couderc C, Besson X, Dayde J, Lummerzheim M, Jacques A (2013) A method to detect and quantify Phaeomoniella chlamydospora and Phaeoacremonium aleophilum DNA in grapevine-wood samples. Appl Microbiol Biotechnol 97:10163–10175CrossRefPubMedGoogle Scholar
  21. Prodorutti D, Pellegrini A, Colombini A, Charlot B, Pertot I (2012) Trichoderma atroviride SC1 is a good wound colonizer and can protect grapevine from infections of Phaeoacremonium aleophilum and Phaeomoniella chlamydospora in nurseries and vineyards. Phytopathol Mediterr 51:447–448Google Scholar
  22. Savazzini F, Longa CMO, Pertot I, Gessler C (2008) Real-time PCR for detection and quantification of the biocontrol agent Trichoderma atroviride strain SC1 in soil. J Microbiol Methods 73:185–194CrossRefPubMedGoogle Scholar
  23. Surico G (2009) Towards a redefinition of the diseases within the esca complex of grapevine. Phytopathol Mediterr 48:5–10Google Scholar
  24. Tegli S, Bertelli E, Surico G (2000) Sequence analysis of ITS ribosomal DNA in five Phaeoacremonium species and development of a PCR-based assay for the detection of P. chlamydosporum and P. aleophilum in grapevine tissue. Phytopathol Mediterr 39:134–149Google Scholar
  25. Waite H, Gramaje D, Whitelaw-Weckert M, Torley P, Hardie WJ (2013) Soaking grapevine cuttings in water: a potential source of cross contamination by micro-organisms. Phytopathol Mediterr 52:359–368Google Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2016

Authors and Affiliations

  • Ilaria Pertot
    • 1
  • Daniele Prodorutti
    • 2
  • Andrea Colombini
    • 1
    • 3
  • Luca Pasini
    • 1
  1. 1.Research and Innovation CentreFondazione Edmund Mach (FEM)S. Michele all’AdigeItaly
  2. 2.Technology Transfer CentreFondazione Edmund Mach (FEM)S. Michele all’AdigeItaly
  3. 3.Cavit S. C. TrentoTrentoItaly

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