Skip to main content
SpringerLink
Log in

Novel bacterial endophytes isolated from winter wheat plants as biocontrol agent against stripe rust of wheat

  • Published:
BioControl Aims and scope Submit manuscript

Abstract

Two strains (JD204 and JC186) were obtained from 127 bacterial endophytes isolated from winter wheat plants, and identified as Pseudomonas putida based on their 16S rRNA and rpoD gene sequences. Both strains produced indoleacetic acid (IAA), with the abilities to solubilize inorganic and organic phosphorus, and to promote wheat growth. Strain JD204 had stronger growth-promoting, phosphorus-solubilizing and IAA-producing activities than strain JC186. Natural infection of stripe rust on the JD204-inoculated winter wheat cultivars was investigated. Disease incidence and disease index were significantly reduced on seven and eight out of 11 JD204-treated wheat cultivars, respectively, compared to their respective controls. Yields were significantly enhanced on eight out of 11 wheat cultivars treated by the strain compared to the controls. The results indicated that strain JD204 could be considered a promising biocontrol agent against stripe rust as well as a yield-enhancing agent for ecofriendly wheat production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Abraham A, Philip S, Jacob CK, Jayachandran K (2013) Novel bacterial endophytes from Hevea brasiliensis as biocontrol agent against Phytophthora leaf fall disease. BioControl 58:675–684

    Article  CAS  Google Scholar 

  • Ajaz M, Khaliq N, Saleem F, Rasool SA (2013) Production and characterization of a bacteriocin-like inhibitory substance produced by indigenous soil associated Pseudomonas putida MAS-1. J Chem Soc Pak 35:976–982

    Google Scholar 

  • Arunarani A, Chandran P, Ranganathan BV, Vasanthi NS, Khan SS (2013) Bioremoval of basic violet 3 and acid blue 93 by Pseudomonas putida and its adsorbtion isotherms and kinetics. Colloids Surf B Biointerfaces 102:379–384

    Article  CAS  PubMed  Google Scholar 

  • Buchanan RE, Gibbons NE (1984) Bergey’s manual of determinative bacteriology, 8th edn. The Williams & Wilkins Company, Baltimore

    Google Scholar 

  • Carleton MA (1915) A serious new wheat rust in this country. Science 42:58–59

    Article  CAS  PubMed  Google Scholar 

  • Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337

    Article  Google Scholar 

  • Colyer PD, Mount MS (1984) Bacterization of potatoes with Pseudomonas putida and its influence on postharvest soft rot diseases. Plant Dis 68:703–706

    Article  Google Scholar 

  • Dong XZ, Cai MY (2001) Common bacterial systematic identification manual. Science Press, Beijing

    Google Scholar 

  • Ebrahimi P, Plettner E (2014) Biodegradation of 1-allyloxy-4-propoxybenzene by selected strains of Pseudomonas putida. Biodegradation 25:31–39

    Article  CAS  PubMed  Google Scholar 

  • Enjalbert J, Duan X, Leconte M, Hovmøller MS, Vallavieille-pope CDE (2005) Genetic evidence of local adaptation of wheat yellow rust (Puccinia striiformis f.sp. tritici) within France. Mol Ecol 14:2065–2073

    Article  CAS  PubMed  Google Scholar 

  • Eriksson J, Henning E (1896) Die Getreideroste. Ihre Geschichte und Natur sowie Massregein gegen dieselben. P. A. Norstedt and Soner, Stockholm

    Google Scholar 

  • Flaishman MA, Eyal Z, Zilberstein A, Voisard C, Haas D (1996) Suppression of Septoria tritici blotch and leaf rust of wheat by recombinant cyanide-producing strains of Pseudomonas putida. Mol Plant Microb Interact 9:642–645

    Article  CAS  Google Scholar 

  • Gardner JM, Feldman AW, Zablotowicz RM (1982) Identity and behavior of xylem-residing bacteria in rough lemon of Florida citrus trees. Appl Environ Microb 43:1335–1342

    CAS  Google Scholar 

  • Germida JJ, Siciliano SD (2001) Taxonomic diversity of bacteria associated with the roots of mordern, recent and ancient wheat cultivars. Biol Fert Soils 33:410–415

    Article  Google Scholar 

  • Gordon SA, Weber RP (1951) Colorimetric estimation of indoleacetic acid. Plant Physiol 26:192–195

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guo C, Yang JG, Shen LL, Qian YM, Huang J, Jiang JF, Wang FL (2011) Screening and identification of Pseudomonas putida strain against TMV. J South China Agric Univ 32:57–60

    Google Scholar 

  • Hassan MN, Afghan S, Hafeez FY (2010) Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria. BioControl 55:531–542

    Article  Google Scholar 

  • Hu N (2006) Isolation and chracterization of a cadmium resistant Pseudomonas putida and cloning of key genes involved in cadmium resistance. Wuhan: Doctor Dissertation of Huazhong Agriculture University

  • Hu DH, Xu XQ, Wang XS, Fu XJ, Li B, Liu RD (2008) Suppression of Puccinia striiformis f.sp. tritici by the extract of Sophoraf lavescens. Jiangsu Agric Sci (6):120–122

  • Jacobs MJ, Bugbee WM, Gabrielson DA (1985) Enumeration, location, and characterization of endophytic bacteria within sugar beet roots. Can J Bot 63:1262–1265

    Article  Google Scholar 

  • Khan MS, Zaidi A, Wani PA (2007) Role of phosphate-solubilizing microorganisms in sustainable agriculture—a review. Agron Sustain Dev 27:29–43

    Article  Google Scholar 

  • Leboffe MJ, Pierce BE (2010) Microbiology laboratory theory and application, vol 3. Morton Publishing Company, Englewood

    Google Scholar 

  • Li Y, He S, Cao HP, Yang XL (2009) Isolation, identification and growth characteristics of Pseudomonas putida strain M6 with malachite green decolorization. Microbiology 36:57–63

    Google Scholar 

  • Li H, Zhao J, Feng H, Huang L, Kang Z (2013) Biological control of wheat stripe rust by an endophytic Bacillus subtilis strain E1R-j in greenhouse and field trials. Crop Prot 43:201–206

    Article  Google Scholar 

  • Line RF, Chen X (1995) Successes in breeding for and managing durable resistance to wheat rusts. Plant Dis 79:1254–1255

    Google Scholar 

  • Lu R (2000) Chemical analysis methods of agricultural soils. China Agricultural Science and Technology Press, Beijing

    Google Scholar 

  • Lu WH, Xu L, Dai YJ, Yuan S (2005) Screening and identification of a strain for hydroxylation of nicotinic acid. Acta Microbiol Sin 45:6–9

    CAS  Google Scholar 

  • Moreira RR, Nesi CN, De Mio LLM (2014) Bacillus spp. and Pseudomonas putida as inhibitors of the Colletotrichum acutatum group and potential to control Glomerella leaf spot. Biol Control 72:30–37

    Article  Google Scholar 

  • Mulet M, Lalucat J, García-Valdés E (2010) DNA sequence-based analysis of the Pseudomonas species. Envir Microb 12:1513–1530

    CAS  Google Scholar 

  • Nazari K, Torabi M (2000) Distribution of yellow rust (Yr) resistance genes in Iran. Acta Phytopath Entom 35:121–131

    CAS  Google Scholar 

  • Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of host plant root system. Appl Environ Microbiol 68:3795–3801

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pearson CJ, Mann IG, Zhang Z (1991) Changes in root growth within successive wheat crops in a cropping cycle using minimum and conventional tillage. Field Crop Res 28:117–133

    Article  Google Scholar 

  • Sambrook J, Russell D (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Samuel MS, Sivaramakrishna A, Mehta A (2014) Degradation and detoxification of aflatoxin B1 by Pseudomonas putida. Int Biodeterior Biodegrad 86:202–209

    Article  CAS  Google Scholar 

  • Sánchez D, Matthijs S, Gomila M, Tricot C, Mulet M, García-Valdés E, Lalucat J (2014) rpoD gene pyrosequencing for the assessment of Pseudomonas diversity in a water sample from the Wuluwe river. Appl Environ Microbiol 80:4738–4744

    Article  PubMed  PubMed Central  Google Scholar 

  • Sener K (2002) Screening of wheat cultivars for resistance to stripe rust and leaf blotch in Turkey. Crop Prot 21:495–500

    Article  Google Scholar 

  • Shi J, Liu A, Li X, Chen W (2013) Control of Phytophthora nicotianae disease, induction of defense responses and genes expression of papaya fruits treated with Pseudomonas putida MGP1. J Sci Food Agric 93:568–574

    Article  CAS  PubMed  Google Scholar 

  • Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849

    Article  CAS  Google Scholar 

  • Sun D, Yu YS, Yang WB, Tian W, Bai G (2007) Identification and mutation breeding of Pseudomonas putida TS1138 with capability of biosynthesizing l-cysteine from ATC. J Tianjin Univ 40:421–426

    CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Tao A, Pang F, Huang S, Yu G, Li B, Wang T (2014) Characterisation of endophytic Bacillus thuringiensis strains isolated from wheat plants as biocontrol agents against wheat flag smut. Biocontrol Sci Technol 24:901–924

    Article  Google Scholar 

  • Wang Y, Song Y, Zhang T, Liu D, Ran L (2011) Screening of biocontrol agents for suppression of wheat leaf rust caused by Puccinia triticina. J Agric Univ Hebei 34:12–17, 23

    CAS  Google Scholar 

  • Wellings CR, Kandel KR (2004) Pathogen dynamics associated with historic stripe (yellow) rust epidemics in Australia in 2002 and 2003. In: Proceedings of the 11th International Cereal Rusts and Powdery Mildews Conference. August 2004, John Innes Centre, Norwich, UK. European and Mediterranean Cereal Foundation, Wageningen, Netherlands, Cereal Rusts and Powdery Mildews Bulletin, Abstr, A2.74. http://www.crpmb.org/icrpmc11/abstracts.htm

  • Wellings CR, McIntosh RA (1990) Puccinia striiformis f.sp. tritici in Australasia: pathogenic changes during the first 10 years. Plant Pathol 39:316–325

    Article  Google Scholar 

  • Xue AG, Chi D, Zhang S, Li Z (2012) Wheat production and wheat rust management in Canada. J Northeast Agric Univ 19:1–14

    Google Scholar 

  • Yang RD, Wang L, Fan H, Chen F, Su XJ, Chen LJ, Cheng L, Yu HW, Zhong T (2005) Isolation and identification of characteristic of biology of Pseudomonas putida in Grus japonensis. Chin J Vet Sci 41:55–56

    Google Scholar 

  • Ye WB, Fan L (2012) Inhibition effect on wheat stripe rust with extract of Daphne giraldii Nitsche and Xanthium sibiricum Patrin. Biol Disaster Sci 35:373–377

    Google Scholar 

  • Zhang JX, Niu LY, Yu L, Lu L, Zhang HY, Wang FZ, Zhao SS (2008) Research advancement on stripe rust in wheat. Tianjin Agric Sci 14:49–52

    Google Scholar 

  • Zhang W, Chen L, Liu D (2012) Characterization of a marine-isolated mercury-resistant Pseudomonas putida strain SP1 and its potential application in marine mercury reduction. Appl Microbiol Biot 93:1305–1314

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work has been financed by Nanyang Normal University [the projects for supporting the university science and technology innovation team of Henan province (No. 2010JRTSTHNO12) and for the construction of Henan province key discipline in biochemical and molecular biology]. The authors sincerely thank Mr. Rubin Ma (Nanyang National Agricultural Park) for kindly providing the wheat field for the present work.

Author information

Authors and Affiliations

  1. College of Agriculture, Guangxi University, Nanning, 530007, China

    Fahu Pang & Gongming Yu

  2. School of Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China

    Fahu Pang, Tan Wang, Chenchen Zhao, Aili Tao, Ziwei Yu & Siliang Huang

Authors
  1. Fahu Pang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chenchen Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aili Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ziwei Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Siliang Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gongming Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Siliang Huang.

Additional information

Handling Editor: Monica Hofte.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pang, F., Wang, T., Zhao, C. et al. Novel bacterial endophytes isolated from winter wheat plants as biocontrol agent against stripe rust of wheat. BioControl 61, 207–219 (2016). https://doi.org/10.1007/s10526-015-9708-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10526-015-9708-x

Keywords

Search

Navigation