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BioControl

, Volume 60, Issue 3, pp 363–373 | Cite as

Isolation and characterization of a Lecanicillium psalliotae isolate infecting cardamom thrips (Sciothrips cardamomi) in India

  • C. M. Senthil KumarEmail author
  • T. K. Jacob
  • S. Devasahayam
  • Sharon D’Silva
  • N. K. Krishna Kumar
Article

Abstract

Cardamom thrips (Sciothrips cardamomi Ramk.) (Thysanoptera: Thripidae) cause serious economic loss to cardamom, Elettaria cardamomum (L.) Maton (Zingiberaceae). A fungus was isolated from cadavers of cardamom thrips collected during a survey in cardamom plantations of Wayanad District, Kerala, India and identified as Lecanicillium psalliotae (Treschew) Zare & W. Gams (Ascomycota: Hypocreales) based on morphological and molecular studies. Bio-assay studies with purified conidial suspension further confirmed the infectivity of the fungus to thrips. At the highest dose tested (1 × 107 conidia ml−1), up to 62.9 % mortality was recorded in the test population, ten days post inoculation. The fungal pathogenesis on adult thrips was documented by scanning electron microscopic studies. This is the first record of L. psalliotae in India and also a first report of a fungus infecting cardamom thrips.

Keywords

Biological control Elettaria cardamomum Electron microscopy Entomopathogenic fungi Lecanicillium psalliotae Sciothrips cardamomi 

Notes

Acknowledgments

The work was carried out under a grant of ICAR under an Outreach Programme on Sucking Pests of Horticultural Crops. We thank Director, IISR for necessary facilities, Dr. Rasoul Zare, Iranian Research Institute of Plant Protection for relevant literature and Dr. R. Ramakrishnan Nair for photomicrograph facility. Technical help extended by Distributed Information Sub-Centre, K.K. Sasidharan, K. Jayarajan (IISR) and K. B. Rebijith (IIHR, Bengaluru) are greatly acknowledged. Thanks are due to Dr. A. Dhandapani, National Academy of Agricultural Research Management, Hyderabad for statistical analysis. We thank the anonymous reviewers and editor for their valuable comments which helped to improve the manuscript.

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Copyright information

© International Organization for Biological Control (IOBC) 2015

Authors and Affiliations

  • C. M. Senthil Kumar
    • 1
    Email author
  • T. K. Jacob
    • 1
  • S. Devasahayam
    • 1
  • Sharon D’Silva
    • 2
  • N. K. Krishna Kumar
    • 3
  1. 1.Division of Crop ProtectionIndian Institute of Spices ResearchKozhikodeIndia
  2. 2.Cardamom Research CentreMadikeriIndia
  3. 3.Indian Council of Agricultural ResearchNew DelhiIndia

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